Enzyme Activity Essay

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LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity Khadija Mohamed Dr Smith December 10, 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) present; sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase as glucose is a product produced by sucrase activity. 2. Explain why denatured sucrase was used as a control. to determine if a true effect was made on the active sucrase by ph, temperature and sucrose or if affect would be seen on both denaturated and active sucrase 3. Alkaline DNS denatures sucrase. Explain why it is important to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the enzyme

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