Introduction In this lab, we performed three titrations to determine the mass of ASA in a caplet through the process of diluting the caplet with a solution of NaHO and isopropyl alcohol. The mixed solutions gave us a double displacement reaction. Double displacement: AB+CD = AD+CB Example: AgNO3 + NaCl = AgCl +NaNO3 This is a double displacement reaction. In the titration of HC9H7O4 = NaC9H7O4 + H2O A titration is a process in which a solution is added to another solution, such that it reacts under conditions in which the added volume may be accurately measured. Three titrations were performed to find the caplets mass.
This representation reflects the fact that there is a hybrid ion represented by the H in NADH and one hydrogen atom represented by H+. The hybrid ion is passed as an electron from complex to complex in the ETC, while the hydrogen atom is shuttled by each of the complexes into the intermembrane space to create a hydrogen ion gradient. This gradient will later be used by oxidative phosphoralation to create ATP. NADH with H+ enters the ETC via the NADH dehydrogenase complex (A.K.A Complex 1). NADH dehydrogenase removes the electrons with the hydrogen atom from NADH.
After waiting a minute, 50 µL of pyruvate was then added to the cuvette and then mixed. The cuvette was then placed in a spectrophotometer and the initial absorbance of the solution was measured. Subsequent absorbance measurements were taken each minute over a three minute period. The overall enzyme activity was measured indirectly through the absorbance of the solution. Absorbance was measured using a spectrophotometer, an instrument that measures the light absorption of a solution at a given wavelength with a spectrophotoelectric cell.
3. Make three sets of the six test tubes and place a couple in the three different beakers 4. Place test tube 1 and 2 in the beaker that has a temperature of 0-10degrees. 5. Place test tube 3 and 4 in the beaker that has a temperature of 35-40degrees.
I added varying levels of substrate to the test tubes in each experiment. The amount of substrates were .5 grams, 1 gram, 2 grams, 4 grams, and 8 grams. The output of the experiment (the dependent variable) was the number of molecules of product formed per minute at (106). RESULTS Test Tube # | pH Level | Amount of Substrate | Number of Molecules of Product Formed per Minute (106) | Test Tube #1 | 3 | .5g | 19 | Test Tube #2 | 3 | 1.0g | 39 | Test Tube #3 | 3 | 2.0g | 82 | Test Tube #4 | 3 | 4.0g | 96 | Test Tube #5 | 3 | 8.0g | 96 | | | | | Test Tube #1 | 5 | .5g | 39 | Test Tube #2 | 5 | 1.0g | 81 | Test Tube #3 | 5 | 2.0g | 168 | Test Tube #4 | 5 | 4.0g | 198 | Test Tube #5 | 5 | 8.0g | 198 | | | | | Test Tube #1 | 7 | .5g | 72 | Test Tube #2 | 7 | 1.0g | 145 | Test Tube #3 | 7 | 2.0g | 300 | Test Tube #4 | 7 | 4.0g |
I then measured my breathing rate by counting how many breaths I took in within thirty seconds, when thirty seconds had passed I stopped measuring my breathing rate and wrote it down, after this I then multiplied it by two in order to get how many breaths I took per minute. 3. After this I then took my temperature using a thermometer and recorded the result on a piece of paper. 4. I then carried out an exercise where I walked up six flights of stairs and then returned back to the classroom.
INTRODUCTION: This experiment aims to demonstrate liquid-liquid extraction. Chemicals used were 18.0 M Acetic Acid, Toluene, Phenolphthalein and 0.1M Sodium Hydroxide. In this experiment, the process of titration was used in order to determine the amount of acetic acid, which was done in three different procedures (1 trial each). The calculations took place right after accomplishing all
Two drops of water were added to the cooled crucible. The crucible with lid completely on it was placed above the clay triangle. The product was reheated for three minutes. The crucible and product was heated for about five to eight minutes. BE SURE THE CRUCIBLE IS COOL.
Lastly, the final product was analyzed by mass spectrometry and HNMR. Reaction setups: Drawing of the new apparatus is attached. Spectra: Please see attached GC-MS and HNMR. Calculations: Mechanism: Conclusion: The hydroboration-oxidation reaction is a reaction that is used to form an alcohol from an alkene. In this reaction there were two isomers of an alcohol formed.