Effects of Enzyme Activity

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Effects of Volume on Enzyme Activity Biology 103 Second Spring Semester Introduction Enzymes are biological catalysts that catalysis biochemical reactions in living calls. The purpose of a catalyst is to decrease the activation energy required for a reaction to happen naturally. Enzymes increase the reaction rate by molecules by two hundred million times faster opposed to if there no enzyme present. During a catalyzed reaction, a substrate binds to an active site which in-turn forms an enzyme-substrate complex. This is where the reactions occurs. From there the enzyme breaks the bond in the substrate and the enzyme can continue to repeat the process. Catalase is an enzyme found in almost all living organisms. In the human body it is an enzyme that is produced by our liver to break down hydrogen peroxide into water and oxygen. Even though hydrogen peroxide is naturally produced by our body as a byproduct of our metabolic process, it is very toxic to our body. Catalase helps to break down hydrogen peroxide so that our body can process it easier. The purpose of this experiment is to determine the amount of oxygen gas produced when different amounts of catalase are introduced to hydrogen peroxide under the same conditions. Hypothesis The amount of gas produced will increase based on the amount of catalase added to the hydrogen peroxide. If more catalase added to the same amount of hydrogen peroxide at the same temperate, the more oxygen gas will be produced. Materials * 3 Test Tubes * 3 Balloons * 27 mL of Hydrogen Peroxide * Measuring Spoons (1/4 TSP, 1/2 TSP, 1 TSP) * Permanent Marker * Ruler * 20 cm String * Test Tube Rack * Thermometer * Yeast Packet * Hot water * Stopwatch Method 1. Use permanent marker to label test tubes 1, 2, and 3. 2. Fill each test tube with

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