The Effect Of Ph On Enzyme Activity

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The Effect of pH on Function of Catecholase Enzyme in Potato Abstract In biochemical reactions, enzymes activity could be changed based on these factors: concentration of substrates, salt concentration, allosteric regulators, temperature and pH. Inventing the hypothesis is increasing concentration of pH would increase the activity of enzyme Catecholase and the rate of reaction. Therefore, the purpose of this experiment was to test the effect of pH on Catecholase (one kind of enzymes in potato) activity by using pH12 buffer. By using the Spectronic 20D+ Spectrophotometer, the change in absorbance over time can be recorded. The result proved: Contract to the hypothesis, at pH 12, activity of enzyme Catecholase and the rate of reaction decreased. Introduction pH is a measure of the concentration of hydrogen H+ in solution so the higher concentration hydrogen ion, the lower pH. pH is one of the most important factors affect to human body. pH was divided three parts on pH scale from pH 0 to pH 14: at pH 7, solutions are neutral. pH > 7 is alkalinity environment that will bind hydrogen ion, and pH < 7 is acidic environment that will donate hydrogen ion. On the other hand, increasing hydrogen ion concentration will reduce the bound metal cation concentration. Decreasing hydrogen ion concentration will increase the hydroxyl ion concentration (M. Chaplin, 2004). Enzymes are one of the most efficient catalysts in biochemical reactions. They are used to speed up the reaction rates by providing an alternative reaction pathway of lower activation energy, which is the minimum energy that requires for reactions occur. Catalysts take part in a reaction (chemical involved), but they do not undergo any changes in chemical reaction. (Farabee, 2001) Enzymes are proteins made of a combination of different amino acids. Therefore, the enzymes may be lost
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