Drosophila Sin3 Essay

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DEVELOPMENTAL DYNAMICS 237:3040 –3050, 2008 PATTERNS & PHENOTYPES Drosophila SIN3 Is Required at Multiple Stages of Development Vishal Sharma,† Aishwarya Swaminathan,† Riyue Bao, and Lori A. Pile* SIN3 is a component of a histone deacetylase complex known to be important for transcription repression. While multiple isoforms of SIN3 have been reported, little is known about their relative expression or role in development. Using a combination of techniques, we have determined that SIN3 is expressed throughout the Drosophila life cycle. The pattern of expression for each individual isoform, however, is distinct. Knock down of all SIN3 expression reveals a requirement for this protein in embryonic and larval periods. Taken together, the data suggest that SIN3 is required for multiple developmental events during the Drosophila life cycle. Developmental Dynamics 237:3040 –3050, 2008. © 2008 Wiley-Liss, Inc. Key words: Drosophila; SIN3; deacetylation; transcription Accepted 17 July 2008 INTRODUCTION Development of multicellular organisms requires coordinate spatial and temporal regulation of gene expression. Levels of gene expression have long been correlated with the amount of histone acetylation (Allfrey et al., 1964). Overall acetylation levels are regulated by the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) (Kuo and Allis, 1998). Mutations in HATs and HDACs lead to developmental defects and/or lethality in a variety of metazoans, likely due to altered expression of genes encoding key developmental regulators (Lin and Dent, 2006). For example, Drosophila melanogaster reared on food containing the HDAC inhibitor trichostatin A (TSA) exhibited a developmental delay at low concentrations and lethality at the highest concentrations tested (Pile et al., 2001). Multiple HDACs are present in Drosophila. SIN3 is a

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