Drosophila Crosses 1 and 2

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Writing Assignment 4 Name: Michael Tran FlyGene Unknown Code: 230 PS ID #:1109766 Lab Section: 16241 BIOL 3311 - Spring 2012 TA Instructor Name: Sneha Koneru Date: March 29, 2012 Materials and Methods In order to contain and cultivate Drosophila melanogaster flies, 25 x 95 mm polypropylene vials are used and are enclosed with foam stoppers. Provisions are made from a dehydrated cornmeal-based formula called 4-24 Blue and is prepared by combination with diluted water. The appropriate ratio of fly food is obtained by measuring half of the provided metal food cup with 4-24 Blue and mixing it with 7 ml of diluted water using a 15 ml conical test tube. Once complete, four granules of dry baker’s yeast are placed on the hydrated food as a nutritional supplement and to prevent the growth of bacteria. To manipulate the flies, they are anesthetized using CO2 that is administered through the FlowBuddyTM device with a 5 L/min flow rate. Once immobile, the flies are examined and scored under a 10.5-45X magnification stereomicroscope. The flies are returned to the incubation chamber set at 25ºC to facilitate optimal growth conditions. The first discriminant cross, DC1, is made by crossing mutant virgin females with Curly/Plum; Dichaete/Stubble marker males. Depending on the genetic outcome of DC1, the second discriminant cross, DC2, is made by crossing female wild-type virgins with F1 males if the unknown mutation is dominant, or by crossing F1 males with mutant females if the unknown mutation is recessive. Statistical analyses on these crosses are performed by determining the chi-square values to test for the accuracy of the hypothesis. The null hypothesis is rejected if the chi-square value is lower than critical values on the chi-square distribution table. Results First Genetic Cross: Discriminant Cross 1 The mode of inheritance for the

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