Dna Fingerprinting Lab Report

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DNA Fingerprinting: Revealing the Truth Name: Zainab Khatoon Due Date: March 19th, 2012 BSC2010L.019S12 Course Name: Biology I Cellular Processes Lab Section: 019 Lab Partner: Hiba Fatima Materials and Methods Restriction Enzyme Digestion Procedure: As with all experiments done in this lab, preparation was accounted for with gloves and goggles. To begin the restriction enzyme digestion procedure, microtest tubes were labeled 1 through 4 for different reactions of restriction enzyme digestion. Then, with the use of the P20 micropipette, ten microliters of Enzyme Reaction Buffer were dispensed into each of the reaction tubes. After the Buffer was dispensed, a fresh micropipette tip replaced the old one. The tip of the micropipette…show more content…
However, for this procedure it was important to make sure that when the gel tray was repositioned, the row of wells was located at the negative pole once the apparatus was connected to the current. Then, the electrophoresis apparatus was filled with 250 mL of 1X TBE buffer so that the surface of the gel was submerged. The gel comb was then carefully removed. Thirty microliters of each of the DNA samples were loaded as depicted in Table 2. Once the DNA samples were loaded, the apparatus was covered and snapped into place. The black wire was inserted into the black input of the power source. The red wire was inserted into the red input of the power source. The power source was set to run the gel at 150V and 150 mA for 40 minutes. After the electrophoresis was completed, the power was turned off, the power source was unplugged, and the leads were disconnected so that the cover could be…show more content…
Discussion As previously mentioned, the results indicate that there was a match in the second, fourth, and sixth lanes. All three of these lanes contained DNA that was cut with restriction Enzyme 1. This data tells us that both suspect 1 and suspect 2 could have been present at the crime scene. However, when we observed the band patterns for crime scene 2, there was only one matching band pattern for lane three: lane seven. Unlike lane five and seven, which also contained Enzyme 2, there was a perfect match. This means that suspect 2 was present at the crime scene according to the match. If the second Enzyme was not used, then the results from the first Enzyme would mean that both suspects were guilty of the crime which may have falsely accused a suspect. Enzyme 2 helped narrow down the suspect and discriminate the true criminal. The data indicates that suspect two was definitely present at the crime scene. However, this does not automatically mean that suspect 2 is guilty of the crime. The suspect could have been at the same location at a different time than when the crime was committed. Further analysis must be done to confirm who the true criminal

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