Purpose (5 points): The purpose of this lab is to learn how to extract DNA and to analyze extracted DNA. This lab allows the conductor of the lab to analyze the steps taken to extract the DNA and realize the purpose of each step. This lab activity teaches one how cell barriers can be broken. Hypothesis: If the enzyme, alcohol, detergent, alcohol, and salt are all used accordingly to extract the DNA from the split peas, then a small amount of the DNA will separate from the solution, looking like long thin strands. DNA is insoluble in alcohol, but soluble in water, so this experiment will test this scientific principle of alcohol.
Put 15 drops of dilute (6 M) sodium hydroxide (NaOH), into each test tube and allow them to stand for at least 1 minute. This prepares the glass surface for the Tollens’ test. **Safety alert** Dilute NaOH should be used carefully. It attacks the skin and is especially damaging to the eyes. If any gets on the skin or in the eyes, wash it off immediately with cool water.
This experiment will help the researcher understand the effect that home solutions such as salt and sugar have on waters freezing point. The plan of the project plan is to start off by collecting all the materials needed in order to complete the experiment, next step to come up with a hypothesis, after creating a hypothesis a procedure must be used to carry out the experiment. After completing the experiment the last step would be to create a graph using the results from the experiment. Research was done in order to find does the amount of salt and sugar effects the freezing point of water? The concept of freezing point depression is helpful in applications where water needs to be kept in a liquid state.
Use the pipet to place 10ml of water into an empty test tube 3. Place two drops of red dye into the test tube 4. Place one drop of yellow dye into the test tube 5. Compare the controlled color to the new color 1. Repeat steps one and two 2.
Do not open lid too far. 9.Obtain a 10-mL transfer pipet and a 50-mL buret. 10.Next obtain the 2g of baking soda and place in a beaker. The Na2CO3 solution must be kept in a clean and dry Erlenmeyer flask that is closed because solution will react with water. Doing the Titration 1.Mix 2g of
Goggles and aprons should be worn until the experiment is complete and all solutions are poured down the drain whereas solids should be properly disposed of by being recycled. Materials- * Mixture Sample * 2 beakers * Hot plate * Sieve * Tweezers * Funnel paper/filter paper * Magnets Procedure- Separating the mixture of paper, iron filings, salt, Styrofoam, and sand would be accomplished based on the differences in physical properties of the different components of the mixture. The first step would be to separate the paper from the mixture using a set of tweezers. Then using the magnet wrapped in the plastic lunch wrap move it through the mixture of the remaining solids. The iron filings will stick to the magnet.
Drain cleaner unclogs drains by breaking down the substances caught in the drain due to its acidic quality. Procedure Part I. Litmus Paper One well of the provided well plate was partially filled with one of the given substances, taking special precautions not to spill and ruin the lab. One piece of each color litmus paper was inserted into the substance, first red, and then blue. The results of each paper were recorded individually. After recording the results, the litmus paper should be thrown in the trash.
In the nitration of benzoic acid, the mixture of HNO3 plus H2SO4 and benzoic acid dissolved in H2SO4 was combined. Combining HNO3 and H2SO4 is an acid-base reaction which will be shown in the reactions to be shown. During the dissolution of benzoic acid solids, some unexpected reactions visibly occurred. White slimy and sticky ppts start to form which was unexpected from the reaction mixture. Both mixtures are placed in an ice bath and when it was combined, the temperature of 0-10C was maintained.
Chapter # 9 Column Chromatography Purpose The purpose of this lab is to become familiar with the theory and process of column chromatography as it is used to separate two substances, in this case, Lycopene and β-Carotene. This is done by first extracting lycopene from tomato paste and β-Carotene from carrot puree. First, the each paste is treated with acetone to remove as much water as possible. Then, the extraction is performed with three 5-mL portions of dichloromethane. Each organic extract is then dried over anhydrous calcium chloride pellets and evaporated to dryness.
In the experiment, two beakers were filled with water. One was the control, and consisted of dialysis tubing containing only starch; the other was the experiment and consisted of dialysis tubing containing starch and amylase. Lugol’s reagent was added drop wise into the water surrounding the tubing in both beakers. The beakers were allowed to sit for a short period of time. The solution in the dialysis tubing of the control beaker began to darken into a blue black color as the dialysis tubing is a semi permeable membrane and allows the water and Lugol’s to pass through by diffusion.