Determining The Properties Of An Enzyme

2446 Words10 Pages
DETERMINING THE PROPERTIES OF AN ENZYME I. Abstract Enzymes are responsible for the speed at which chemical reactions they are involved in take place. This experiment determines the effects that concentration, temperature, pH, and boiling have on an enzyme’s ability to perform its work. It is hypothesized that none of these variables will have any effect on the activity of enzymes and these hypotheses are tested using dye-coupled reactions to determine the rate at which peroxidase converts H2O2 into water (H2O) and oxygen (O2). Each hypothesis is subsequently rejected as data suggests that concentration, temperature, pH, and boiling all have an effect on enzyme activity. II. Introduction Part 1: The Effects of Concentration on Enzyme Activity In this experiment, the effects of concentration on enzyme activity were determined by using a spectrophotometer to observe absorbance changes taking place in samples containing a pH 5 buffer, guaiacol, hydrogen peroxide, and different volumes of potato extract. Hypotheses Ho: The amount of enzyme added does not influence the rate of reaction. Ha: The amount of enzyme added does influence the rate of reaction. Part 2: The Effects of Temperature on Enzyme Activity In this experiment, the effects of temperature on peroxidase activity were determined by using a spectrophotometer to observe absorbance changes taking place in temperature treated samples containing potato extract, guaiacol, hydrogen peroxide, and pH 5 buffer. Hypotheses Ho: The temperature of solution does not influence the activity of an enzyme. Ha: The temperature of solution does influence the activity of an enzyme. Part 3: The Effects of pH on Enzyme Activity In this experiment, the effects of pH on peroxidase activity were determined by using a spectrophotometer to observe absorbance changes taking

More about Determining The Properties Of An Enzyme

Open Document