Determination of Calcium by Atomic Emission Spectroscopy

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(hassan, 2014)Determination of calcium by atomic emission spectroscopy Aim: to determine the linear range of the variation in emission intensity with calcium concentration. To quantify the interference by phosphate. To determine the percent recovery of the emission intensity when EDTA is added to a solution of calcium and phosphate. To show the effect of added EDTA on the emission intensity of calcium. Introduction Calcium is a trace element and can be quantified by AES after stock sample solution preparation technique. Flame photometer is adequate because it’s simple for the analysis and calcium atoms are formed and excited at low flame temperatures and emits visible radiation. * Is a diagram relevant? * How does phosphate interfere? * What is EDTA? Ethylene-Diamine-Tetraacetic Acid (EDTA), is a colourless ploy-amino carboxylic acid, it acts as a cover protecting the trace metal calcium from being interrupted by phosphate * How does EDTA reduce phosphate interference? * Are there any other ways of reducing phosphate interference on calcium? * Are there any alternative methods of analysis? Is AES by flame photometer the best choice? Atomic emission spectroscopy by flame photometer is the best choice to conduct this experiment as the atomic emission spectrometry works confining the solution within an emission chamber for on line analysis or individual sample analysis. As calcium atom are formed and excited at low flame temperatures it also releases visible radiation, therefore a simple flame photometer for the analysis can be used. Method: a stock calcium solution was prepared over the concentration ranging from 0-100 µgcm-3 using five volumetric flasks and a bulb pipette. After doing so, used the calcium solution which was prepared to make a varying concentration of phosphate but with the same concentration of calcium. The next step

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