Cytotoxic Activity of Crude Extracts Essay

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UCD web of science, Evidence-Based Complementary and Alternative medicine journal Volume 2011, 7 pages Cytotoxic activity of crude extracts as well as of pure components from Jatropha species, plants used extensively in African medicine Olapeju O. Aiyelaagbe,1 Amao A. Hamid,2 Ernesto Fattorusso,3 Orazio Taglialatela-Scafati, 3Heinz C. Schr¨oder,4 andWerner E. G.M¨uller4 In the 21st century there are many chronic illnesses, one such illness is cancer. Although, there are treatments available for diseases such as cancer there is no guaranteed cure for the millions that it affects globally. A combination of chemotherapy and pharmaceutical medication in general is the most common route followed in the hope of conquering the illness. Of late it has become apparent to those involved in the cancer related industry, that complementary and alternative treatment may well be of benefit. The hypothesis within this article is that the Jatropha curcas plant a member of the Euphorbiacae family, has disease fighting properties in particular cancer fighting properties. Local knowledge of the plants medicinal use was gathered before the authors undertook this experiment; specimens of the plant were collected and authenticated. From the root of the plant crude extracts were obtained with the use of n-hexane, ethyl acetate, and methanol. These crude extracts were subjected to fractionation by column chromatography before being purified. This resulted in 14 compounds being isolated, the extracts were then subjected to an in vitro cytotoxic assay, the MTT [3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltet-razolium bromide] (Sigma Aldrich, Taufkirchen, Germany) method was used. Testing of the extracts was done using three different cell lines; L5178 mouse lymphoma cells, PC12 rat adrenal medulla pheochromocytoma cells, and also hela human cervix cells. The cells were grown in a

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