Do not go into detail about the protocol. The purpose of the experiment was to determine the optimal pH and temperature of a phosphatase enzyme found in a crude protein extract isolated from alfalfa sprouts. The temperature optimum was found to be 55°C, and the pH optimum was found to be 5. Thus, the phosphatase enzyme can be classified as an acid phosphatase. 5.
Experiment 5: Titration for Acetic Acid in Vinegar Name: Lab Partners: None Date of Experiment: March 25th, 2013 Location: My House Course Number: Chem 112 Abstract: In this experiment the objectives were develop familiarity with the concepts and techniques of titration and to determine the concentration of an acetic acid solution in vinegar. Experiment and Observation: Data Table 1: Quantity of NaOH needed to Neutralize 5 mL of Acetic AcidBrand of Vinegar Used: Heinz White Distilled VinegarLabel Notes: ______________________ | | Initial NaOH reading(Interpolate to 0.1 mL) | Final NaOH reading(Interpolate to 0.1 mL) | Volume of NaOH used | Trial 1 | 9.5 | 1.3 | 8.2 | Trial 2 | 9.7 | 1.4 | 8.3 | Trial 3 | 9.6 | 1.4 | 8.2 | Average volume of NaOH used: | 8.2 | Calculations and Errors: Errors: Calculations: A. Calculate the average number of mL of NaOH used for the 3 trials and record. B. Calculate the Normality of the vinegar using the previously given equation.
Juliana Park Mayumi Tamada CHEM 111B LAB/ M-F 1-4PM 15 August 2012 Spectroscopy Lab Introduction In this lab, the molar absorptivity of the complex FeLn2+ will be determined by using the absorbance of the complex and its concentration. The absorbance will be found by using a spectrophotometer. For the next part of the lab, the formula of the complex will be determined by also using the volume of ligand and the absorbance again. Experimental There are two different parts to the experiement. In the first part, five 100 mL flasks of 5 mL ligand solution, 5 mL 2 M sodium acetate, 4 mL 3 M NH2OH, and 1-5 mL Fe2+ solution are diluted with water.
Prelaboratory preparation: Read pages 1 – 30 and 45 – 54 of the lab text. You may disregard the microscale distillation procedures of TECH 0704. Answer in your laboratory notebook the Safety Quiz on pages 11 – 12 and the Prelab Questions 1, 2a, 2b, 3 -5, pp. 67 - 68. EXPERIMENT: TECH 0704, Distillation, macroscale technique only, simple and fractional: Substitute a mixture of 25 mL ethanol and 25 mL water for the toluene/cyclohexane mixture.
Add 5 mL of copper (II) sulphate solution to a test tube. Add 5 mL of sodium carbonate in another test tube. Record the appearance. 2. Write a hypothesis on what you think will happen when mixed.
Coal hypothesis = If coal is introduced to the water, then the ph level will stay the same. 2. Based on the results of your experiment, would you reject or accept each hypothesis that you produced in question 1? Explain how you determined this. a. Pyrite hypothesis accept/reject = I accept my hypothesis because after the pyrite was introduced to the water the level of ph dropped to 4 making the water more acidic.
The purpose of this experiment was to determine the effects of substrate concentration and pH on the initial rate of enzyme-controlled reaction. METHODS I conducted five experiments. In each experiment, I had five test tubes with constant pH levels of an enzyme solution. The pH levels over the 5 experiments were 3, 5, 7, 9, and 11. I added varying levels of substrate to the test tubes in each experiment.
Vinegar, Antacid, and Soap all became positive solutions after I put in the enzyme. A change in pH disrupts an enzyme's shape and structure. pH measure acidity--water is neutral and has a pH of 7. When the pH changes an enzyme's structure, the enzyme can't do its job. Changes in pH break the delicate bonds that maintain an enzyme's shape.
Joel Barlow High School Honors Biology Michael Klein Wassink 11-5-2014 Lab: pH, Buffers & Biological Substances Problem How do organisms survive and function despite metabolic activities that tend to shift pH toward either acidic or basic ends of the scale. Hypothesis I predict that as I add varying amounts of 0.1m HCl and 0.1m NaOH to various biological substances the pH levels of those biological substances will go down with the addition of HCl and up with the addition of NaOH because Acids (HCl) release H+ into substances which makes their pH levels go down, and Bases (NaOH) accept H+ from substances which makes their pH levels go up. Materials • 4 goggles • 4 aprons • 50ml beaker • 1-100ml-grad cylinder • 3-colored pencils • Wide range pH paper • Tap water • Forceps • HCl (0.1M) • NaOH (0.1M) • pH 7 buffer solution • Milk • Potato homogenate • Egg white • Gelatin suspension Procedure 1. Pour 25ml of tap water into the 50ml beaker. 2.
Extraction and Purification of Components in an Analgesic Tablet November 2, 2014 My signature indicates that this document represents my own work. Excluding shared data, the information, thoughts, and ideas are my own, except indicated in the references. 1 Abstract: HPLC is a very important technique used today in many pharmaceutical fields. It helps separate the solutions. After extraction was performed on the tablet it was then ran through an HPLC test.