Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Plant Pigments Depending on Photosynthesis. Is the rate of photosynthesis faster when there are extreme light and temperatures present? Paper chromatography is a technique used for separating and identifying pigments. The solvent DPIP, (2, 6-dichlorophenol-indophenol), which is often used to substitute for NADPH, moves up the paper by capillary action. As the solvent moves up the paper, it carries along any substances dissolved in it, which are the pigments.
Twenty drops of bromothymol blue was also added to the 150mL beaker. The pH was then obtained using the Vernier pH probe and it read 6.68. The 5mL pipet was then used to transfer 5mL of the green solution to the three 50 mL beakers. A 100mL beaker was obtained and filled with 1.0 M HCl solution and the pipet was used to acquire 1mL of the 1.0 M HCl solution. The 1mL of HCl was then transferred to one of the 50mL beakers turning the color of the solution to yellow.
Describe how you carried out your investigation? 1) Add 10cm³ of hydrogen carbonate indicator to each of the 5 boiling tubes 2) Select 5 pond weed shoots of the same length 3) Put pond weed shots in the boiling tube and cover with bong 4) Set boiling tubes up at 20,40,60,80 cm away from your light source 5) Look at the colour of the indicator at 30 minutes 6) convert the colour to PH using a PH spectrum 7) As Co2 is more acidic the more Co2 is used the higher the PH will rise In other words the more photosynthesis the higher the PH. What did you change in your experiment? The light intensity by changing the distance from the lamp What did you keep the same? Why?
Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution). The supernatant was then centrifuged at 9000 x g and 40C for 15 min and 5 ml of the second supernatant was transferred to a conical tube. The obtained second pellet was resuspended in 4 ml of distilled water and transferred into another dialysis
How To Do Paper Chromatography With Leaves By Anne Marie Helmenstine, Ph.D., About.com Guide See More About: * paper chromatography * leaves * plant pigments * pigment chemistry * separation methods You can separate the pigments in colored leaves using chromatography. Try comparing the pigments in different colored leaves or leaves of the same plant collected at different times of the year. Martin Ruegner, Getty Images Most plants contain several pigment molecules, so experiment with different leaves to see the wide range of pigments. Difficulty: Average Time Required: 2 hours Here's How: 1. Take 2-3 large leaves (or the equivalent with smaller leaves), tear them into tiny pieces, and place them into small jars with lids.
Put on a safety goggles. Use a thermometer to measure the initial temperature of the water in the soft drink can. Measure the initial mass on a mass scale. Place the fuel under the soft drink can (If the fuel is half a cashew nut or cheetos stick it to a wine cork using a pin, then wrap the wine cork with a sheet of aluminum foil). Set the stopwatch to 1 minute.
Repeat this two more times 5. Use a pipet to put 10 ml of the appropriate solutions in each bag: Bag 1: 10 ml – 10% sucrose Bag 2: 10 ml – Distilled Water Bag 3: 10 ml – 10% Sucrose 6. Squeeze as much air as possible out of the tubes and tie the remanding open sides shut. 7. Make sure to cut the excess string hanging off of each bag.
In activity 2a, green test tube was clamped to a ring stand, and filled three quarters to the top with 5% sodium bicarbonate. A 10 mL graduated pipette was held upside down and place a short piece of rubber tubing over the delivery end.
Hypothesis When the light is at a closer distance to the Elodea plant, the amount of oxygen bubbles produced in 2 minutes will increase displaying the high rate of photosynthesis. Light is required in order for the process of photosynthesis to function correctly, therefore the higher the light intensity then the rate of photosynthesis will increase too. Variables Independent Variables | The independent variable will be the distance that the light is away from the Elodea plant since this needs to be changed to impact the dependant variable. | Dependant Variables | The