Then again, while leaving behind as much solid as possible, transfer as much liquid as possible into a pressure filtration assembly (p. 68 lab text) and filter the liquid into a clean, dry, tared 25 mL Erlenmeyer flask (tared = weighed empty). Be sure that the filter assembly is resting in the flask prior to transferring the liquid, as some liquid will filter through immediately. The filtration apparatus is made by pushing the micro Buchner funnel (polyethylene frit in place - no filter paper) into the end of a plastic pipet which has had the tip cut off and which has had a small hole cut into the squeeze bulb). Allow most of the liquid to filter by gravity, then, while holding your thumb over the hole in the squeeze bulb, gently apply pressure to complete the filtration. Care must be taken when squeezing the pipet bulb on the filter pipet.
Pour all the filtrate and washings into a 250cm3 volumetric flask. Make up to 250cm3 with 1.0mol dm-3 sulphuric acid(VI) acid. Stopper the flask and invert several times to thoroughly mix the solution. 5. Fill the burette with 0.005mol dm-3 potassium manganate(VII) solution.
Paper chromatography can be used in separating amino acids and anions, RNA fingerprinting, and testing histamines and antibiotics. (Infromation received from sonic.org Purpose The purpose of this experiment was to separate the dyes that these markers are composed of and show how chromatography works. Materials * Four different markers (including one black permanent marker) * Rubbing alcohol or isopropyl alcohol * Coffee filters (2) * Tall glasses or plastic cups (2) * Pencil * Ruler * Tape * Table salt * Water * Measuring cups/spoons * Clean pitcher or 2-liter bottle Procedure 1. Your first task is to cut the coffee filter into a rectangle measuring three cm by nine cm. You will need 2 for this lab.
Transfer the distillate via Pasteur pipet into a 15-mL centrifuge tube. Wash the distillate with two 2.5-mL portions of water, removing and setting aside the water layer at each step. Transfer the organic material to a small, dry Erlenmeyer flask, leaving behind the last drops of the water layer. Remove any drops of water that were accidentally transferred to the Erlenmeyer flask, if any, then add small amounts of anhydrous sodium sulfate as drying reagent. When the liquid is dry, it will be transparent and some of the drying agent will flow freely like beach sand.
The MgSo4 absorbed the last traces of water the ether solution. The solution was completely dry when it appeared crystal clear and MgSO4 floated in the liquid, when swirled. If solution is cloudy, more drops of MgSO4 were to be added until solution was completely dropped. 5) We decanted the solution from the solid MgSO4 into a 50 or 100 ml round bottom flask. 6) The ether was removed using the rotary evaporator.
Abstract The objective of the south street seaweed experiment is to make a tincture of iodine. Iodine is used commercially as an antiseptic on cuts and scrapes of the skin. Conceptually, one of the active ingredients of the tincture, iodide, can be extracted from seaweed. By adding the seaweed to water and applying heat energy into the mixture, we were able to extract iodide. After adding Iodine salts, and filtering the mixture our next goal was to test for three important chemicals that must exist in our mixture for it to be a true iodine tincture; Iodine, Iodide Ion and the triiodide ion.
Enough water should be added so that the flask is full to the 250 ml mark Tightly wrap the top of the flask with a Parafilm when finished 2. Set up the titration apparatus 3. Fill a dry 50 ml buret with EDTA solution using a small
4. The outside of the flask was dried with a paper towel. 5. The stopper was firmly put in the flask. 6.
5a. Place a small amount (the size of an aspirin tablet) of copper II sulfate pentahydrate in a clean dry test tube and record your observations. Using your test tube holder, tilt the test tube and gently heat the contents for 4-5 minutes, while recording your observations. Make sure to observe the upper end of the test tube while heating. Allow the test tube to cool and record your observations.
I added 1 drop of phenolphthalein to wells C1, C2, C3 and C4 and stirred them with a clean toothpick. I then added 5 drops of 1.0 M NaOH, sodium hydroxide solution to wells C1, C2, C3 and C4 and stirred them. Cells 1 and 2 turned a dark pink while cells 3 and 4 were a lighter pink. Cells 1 and 2 would have a shift to the right and be