Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution. Then 5 mL of YY ligand, were poured to each of the five flasks. Each flask had 5 mL of 2M sodium acetate and 4 mL of 3M NH2OH. Then the whole solution was diluted up to the 100 mL fill mark with distilled water. This was the solution that was used in order to obtain the absorption spectrum for each of the different iron (II) ligand examples different flasks.
4. Record the pH measurements in your table. 5. Rinse the beaker thoroughly, and pour into it another 25ml of tap water, and add 0.1M NaOH drop by drop, recording the pH changes in exactly the same way as for the 0.1M HCl. 6.
The sample size was 100 termites and there were 5 replications for each level of treatment. The species being used was termites. The experiment was carried out by first gathering 100 termites into a glass bowl. Two equal sized circles were drawn with each writing utensil on a blank sheet of white paper. Figure 1 shows the circles that were drawn with a red ink pen and red ink sharpie marker.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Take one pipette and fill each test tube with different amounts of Pb 4. Wait 20 minutes 5. Measure the height of the solid in each test tube.
AP LAB 1: Diffusion and Osmosis Exercise 1A: Title: Diffusion Objectives: * To understand the mechanism of diffusion and its significance in cells. * To understand how solute size and concentration gradients effect diffusion across a selectively permeable membrane. * To understand how a selectively permeable membrane between two solutions effects diffusion between them. Hypothesis: If I observe that a Benedict's test has came back positive for glucose in the beaker when it came back negative initially, the color of the bag has been tinted from initially being clear, and the mass of the bag has increased; then I will conclude that the dialysis tubing is permeable to glucose, iodine potassium-iodide, and water but not starch, and diffusion has occurred. Procedure: 1.
The first test tube will be control, the second will be substrate and indicator dye, the third will be dilute extract, the fourth will be the same contents as the second, the fifth will be medium concentration of extract, the sixth will be the same contents as the second, and the seventh will be concentrated extract. 3) Add stock solutions to each tube using the corresponding graduated 5 ml pipette or dispensing device. 4) Adjust the spectrometer to zero absorbance at 500nm. Pour contents of test tube 1 into a cuvette. ) Make sure to keep time, read the spectrometer, and record the data.
Once the solution is clear, retrieve at least ten drops of the solution and place them in a new test tube. 5. Add the same number of drops of NaOH to the solution in the new test tube. 6. If a precipitate forms, record your results including a chemical
Put exactly 5.0 mL of water in the 10.0 mL graduated cylinder. Record this volume in your data table (10.0 mL). Label the first pipet "Acid." To calibrate the pipet, fill it with LIU-2 water. Holding the pipet vertically, add 20 drops of water to the cylinder.
About 50 mg of the powdered mycelium was transferred into a microtube contained 500 µl of TES (100 mMTris, pH 8.0, 10 mM EDTA, 2% SDS). To which, 50 µg proteinase K was added and incubated for 1 h at 60°C with occasional gentle mixing. To the above mixture, 140 µl of 5 M NaCl was added to adjust the salt concentration to 1.4 M. Then 65 µl of 10% CTAB (CetylTrimethyl ammonium bromide) was added and incubated for 10 min at 65°C. To the above mixture, 700 µl of Chloroform and isoamyl alcohol (24:1) was added, mixed gently, incubated for 30 min at 0°C and centrifuged at 10000 rpm for 10 min at 4°C. The supernatant was transferred to a 1.5 ml tube; to which 225 µl of 5 M NH4Ac was added, mixed gently, incubated on ice for 30 min and centrifuged at 10,000 rpm for 15 min at 4°C.