Chromatography Essay

1022 WordsMay 5, 20155 Pages
Chromatographic Analysis Ilagan, Mhariel Anne Denisse, Lacsamana, Marco Sebastian, Lanuza, Reggiana Mae, Latonio, Juan Paulo* Group 6, 2Bio5 Department of Biology, University of Santo Tomas, Manila, Philippines Abstract Chromatography is used to identify different components of certain samples and on plants, their different pigments. In this experiment, samples of common over-the-counter drugs and the sili were used as samples. They had different preparations. The analgesics were subjected to capillary-type of chromatography were the solvent travels up the TLC plate and the sili extract was subjected to column chromatography. The results for the Rf values of SX, Asp, Mef, Para, and Mef are 0.3538, 0.5538, 0.6462, 0.5077, and 0.6615, respectively. This highly suggests that all are of different components. The column chromatography also yielded different pigments such as yellow, orange, light orange, and dark orange, each coming up after the sample was subjected to hexane, hexane-DCM, DCM, and DCM-MeOH, respectively. Introduction There are a lot of drugs and medications for different types of diseases, some may look and smell the same but are totally different. One may be for diarrhea while the other one for migraine. The label on the package of the drug usually tells us what they are and are made of. One can identify the difference of the drugs using chromatography; the collective term for a set of laboratory techniques for the separation of mixtures. The various components of the mixture travel at different speeds, causing them to separate. [1] Chromatography was first employed by Russian scientist Mikhail Tsvet in 1900. He was famous for the actual separation of plant pigments such as chlorophyll, carotenes, and xanthophylls. Since these components have different colors. Further work done by researchers found that the main principles of Tsvet's

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