Cancer is a group of disease characterized by unregulated cell growth and invasion and spread of cells from site of origin, or primary site to other site in the body. And in this previous paper was investigated effect of CAC1 on proliferation of lung cancer A549 cells and activation of signalling pathways of MAPKs. And then was demonstrated that; When ERK ½ is activated in signalling pathway of MAPKs, by promoting tumour agents (PMA) CAC1 promotes the cell proliferation in lung carcinoma A549 cells.
What is siRNA? we can explain to si RNA due to figure like that figure; if you want and then we can say two key approaches to RNA that have gained substantial interest for use gene silencing are the doubled stranded small interfering siRNA and vector based short hairpin (shRNA) ,While both siRNA and short RNA (FIGURE ) can be used for protein knockdown. If you turn the study paper we can see siRNA result in CAC1 siRNA. Furthermore siRNA causes leading to cell death (apoptosis).In this paper showed, CAC1 siRNA enhanced the apoptosis in A549 adenocarcinoma cells.
We can explain what is CAC1 gene?
CAC1 gene which is novel gene, contains cullin and belong to cullin family of E3 ubiquitin ligases and takes place in MAPKs signalling pathway.
Mitogen-activated protein kinase (MAPK) modules containing three sequentially activated protein kinases are key components of series of vital signal transduction pathways regulate processes such as cell proliferation, cell differentiation and the cell death (apoptosis) in eukaryotes from yeast to human.MAPKs phosphorylates diverse substrates in the cytosol and nucleus to bring about changes in protein function and gene expression that execute the appropriate biological response.
And we can put MAPKs pathway in the power point. Because that picture is indicating ERK1/2 and p38 MAPKs.
Normal lung tissue, lung carcinoma and A549 cells were treated with 60nm siRNA...