Catalase Lab Report Experiment.

1161 WordsJun 26, 20115 Pages
'How temperature affects the rate of reaction of Catalase on the breaking down of Hydrogen Peroxide.' Introduction 'How temperature affects the rate of reaction of Catalase on the breaking down of Hydrogen Peroxide.' Enzymes are catalysts. Some enzymes are proteins. The job of an enzyme is to break down substrates, and in return a product is released from the active site (area where the breaking down happens) of the enzyme. From one substrate two products are released. Image 1_Enzyme/Substrate The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide, into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2. There are limiting factors which if altered, can alter the procedure of the reaction, such as temperature, pH, and the concentration of either the enzyme or the substrates. If these are altered a variety of results will occur. This variety of results allows to find the optimum point for the enzyme to break down the substrates, the faster the better. The objective for this experiment is to find the optimum temperature for Catalase to break down hydrogen peroxide. 'How temperature affects the rate of reaction of Catalase on the breaking down of Hydrogen Peroxide.'(1) Image 2_Hydrogen Peroxide/Catalase This image explains what will happen in between the Catalase and the Hydrogen Peroxide. In situation A) The hydrogen peroxide is the substrate and its going to go to the Catalase which is the enzyme to be catalised. In B) The reaction is being consumed, as the hydrogen peroxide has fit into to the Catalase as a key in a lock, leading to situation C) where the reaction has finished, and so Oxygen and Water were the products of the reaction. Hypothesis The expectations for this experiment is that with the temperature too low the enzyme will denature

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