Catalase Hsc Assessment

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Catalase introduction Enzymes are Catalysts; they are not used up in the reactions or changed in the reactions. Cells use enzymes to break down poisonous chemicals into harmless substances. Enzymes can be very sensitive to changes in pH and temperature Enzymes are proteins, Catalase is an enzyme found in the cells of many living tissues, including potato. It speeds up a reaction that breaks down hydrogen peroxide. Hydrogen peroxide is a poisonous chemical to all living things. Catalase converts H
2O
2into 2 separate harmless substances, water and oxygen. In this experiment we will be testing the effects of temperature, pH and substrate concentration on Catalase. The optimum pH of catalase is 7 and its optimum temperature is 37 degrees. Aim: To test the effect of change in pH, Temperature and substrate concentration on catalase in liver Hypothesis: we predict the enzyme will have its greatest reaction when it is in its optimum temperature, pH and substrate concentration Risk assessment: * Wear gloves when handling raw liver. * Be careful of glass and sharp objects such as scalpel. * Wear safety glasses when observing reaction. * Beware of boiling water and Bunsen burner * Alert your teacher to any spills or accidents * Handle Hydrogen peroxide with care All Materials: * Liver (100g) * Scalpel * 3x large beakers * Ruler * Bunsen burner * 1 molar hydrogen chloride- dropper bottle * Gauze mat * Thermometer * 14 x test tubes * Test tube holder * 1 molar Hydrogen peroxide- dropper bottle * Universal Indicator * pH colour chart * Water * Sodium hydroxide * 2x plastic gloves * Safety glasses * Ice (one large beaker full) Method: A) Testing the effects of Increased temperature 1. Gather the materials and set up all equipment 2. With a scalpel

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