Catalase Enzyme Test (11th Grade Experiment)

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The experiment that I have undertaken is an enzyme lab that will be testing the reaction speeds of the enzyme catalase in different concentrations of Hyodrogen Proxide (H2O2). This is so that we can see how our body breaks down H2O2, a poisonous chemical that, if left by itself in our body, would kill us. The experiment is based off the latter part of AP Biology Lab 2: Enzyme Catalysis by Bozeman Science, which in the beginning he explains how enzymes work, and later uses Catalase as an example. The specifics of the experiment will be based off This experiment is important to do because we must understand the way enzymes work in our own body, and so would be important to medical researchers trying to see how different things affects the body. Research Question: What is the effect of H2O2 concentration on float speed of Catalase soaked coffee filter? Hypothesis. Catalase is an enzyme that digests Hydrogen Peroxides into water and oxygen. Since the speed of an enzyme reaction relies on whether there is any substrate to react with, float speed should increase as substrate concentration increases. Therefore, if H2O2 concentration increases, float speed will increase. If H2O2 concentration decreases, float speed will decrease. This hypothesis is based off where substrate concentration is seen to increase rate of reaction. It can be noted however that rate of reaction will level off after a certain concentration level, which can be called Vmax, or maximum velocity of reaction. Table: | #1 | #2 | #3 | #4 | IV Hydrogen Peroxide Percentage | 0.00% | 0.10% | 0.20% | 0.30% | CV1 Temperature | 30˚C | 30˚C | 30˚C | 30˚C | CV2 Enzyme concentration | 1 Unit | 1 Unit | 1 Unit | 1 Unit | CV3 pH | 8 | 8 | 8 | 8 | CV4 Reaction volume | 30 mL |

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