Caries Activity Test

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CARIES ACTIVITY TEST * Caries activity test are primarily undertaken to evaluate the susceptibilty of the individual towards caries USES OF CARIES ACTIVITY TEST * FOR CLINICIAN * To determine the need for caries control measure * Aid in determining the result of the preventive measures undertaken * Orthodontics can be cautioned during the treatment * FOR RESEARCH WORK * Aid in selection of cases for the study of caries * Help in screening of patented therapeutic agents * Serves as an indicator of period of exacerbation * THE BASIS OF THIS TEST IS * The oral micro-organism and fermantable carbohydrates are causative agents in dental caries * The retention of carbohydrates in mouth favour the growth of micro-organism LACTOBACILUS COUNT TEST * It evaluates lactobacilli bacteria in the saliva quantitatively. * NUMBER OF LACTOBACILLI AND CARIES ACTIVITY * NUMBER CARIES ACTIVITY * 0-1000 + little or no caries * 1000-5000 ++slight caries * 5000-10000 ++moderate caries * 10000 and more +++marked caries The patient is given sterile bottles and pices of paraffin to chew He is instructed to collect saliva early morning for three successive days before brushing and taking food Chewing paraffin will stimulate enough quantity of saliva The collected saliva is thoroughly stirred and 1.0ml of saliva is mixed with 4ml of beef adjusted at ph 5 0.1ml of diluted solution is then transferred to diluted tomato juice agar to which 1:10000 sodium has been added * The solution is distributed evenly with a glass spreader * The medium is incubated at 98.6F(37c) for 72 hrs after which colonies are counted * The solution is distributed evenly with a glass spreader * The medium is incubated at 98.6F(37c) for 72 hrs after which colonies
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