K+ Perform flame test by dipping a test loop in test solution and putting in Bunsen flame after cleaning the loop with HCl(aq). Observe flame through a cobalt blue glass. A lavender flame occurs and the flame is observable through the cobalt blue glass. Presence of potassium in the test solution - 3. NH4+ Heat 5mL of the test solution in a beaker.
Add color indicator 4. Obtain a burette and fill it with NaOH 5. Titrate the 10 mL with NaOH until it turns pink 6. Weigh a dry funnel with a filter paper and record the weight 7. Obtain an Erlenmeyer flask that has a vacuum opening and attach the vacuum tube to it 8.
To observe the change in properties of various synthesized copper compounds in order to identify the reactions and products being formed. Secondly, to become familiar with methods of separating compounds through decanting, heating with Bunsen burner and suction filtration system. In doing so, be able to recover copper metal and interpret the resulting percentage yield by applying the law of conservation of matter. Upon combining 20.0mL of NaOH to 10.0mL of Cu(NO3)2 and stirring the solution, there was a darkening of the initial blue color. Adding distilled water to the solution lowers the initial intensity of the blue color, bringing back to a lighter blue.
Approximately 20 drops of a .04% Bromothymol blue solution was then added to the beaker of the phosphate buffer. Using a clean 5 mL serological pipette, transfer 5 mL of the phosphate buffer and Bromothymol blue solution to each of three clean 150 mL beakers. Next, using the buret for HCl, add 1 mL of HCl to one of the three beakers, then label this beaker “Yellow”. Next use the buret for NaOH and add 1 mL of NaOH to another of the three beakers, and label this beaker “Blue”. Lastly add 1 mL of water using the buret for water to the last beaker, and label this beaker “Green”.
This is the molecule which makes photosynthesis possible, by passing its energized electrons on to molecules which will manufacture sugars. All plants, algae, and cyanobacteria which photosynthesize contain chlorophyll "a". A second kind of chlorophyll is chlorophyll "b", which occurs only in “green algae” and in the plants. Bibliography: http://www.ucmp.berkeley.edu/glossary/gloss3/pigments.html Conclusion: I learned various things from this lab. For instance, I learned how pigments absorbed strongly move slowly and pigments absorbed weakly move the fastest.
These amazing organisms are capable of capturing the energy of sunlight and fixing it in the form of potential chemical energy in organic compounds.” (Measuring the rate of photosynthesis) “Light is one of the main factors that affects the rate of photosynthesis, which literally means using light to create something new.” (What Are Three Factors That Affect the Rate Which Photosynthesis Occurs?) This experiment was to compare how the Elodea photosynthesized when absorbing different colors of light, and to measure the absorbance from 500 nm of wavelength to 750 nm. Based on three types of pigments that are present in plants: chlorophyll, carotenoids, and phycobilins, it was predicted that both of the rates of photosynthesis and the absorbance of different wavelengths were high under the colors of violet-blue or orange-red, while rates were low under the light color of green-yellow. Materials and Procedures: The materials used for this experiment were as follows: Elodea leaves (that have been kept in the dark), spinach leaves, blender, cheesecloth, spectrophotometer, cuvettes, beakers, large test tube, 5% sodium bicarbonate, Kimwipes, razor blade, tape, 100 mL graduated cylinder, graduated pipettes with rubber tubings, light source, ring stands and clamps, cellpphane paper. In activity 2a, green test tube was clamped to a ring stand, and filled three quarters to the top with 5% sodium bicarbonate.
The energy needed for photosynthesis comes from sunlight, which is the variable for this experiment. The substance that absorbs sunlight is chlorophyll, which is mainly contained in chloroplasts. This energy is used to convert carbon dioxide (CO2) and water into sugars. This conversion creates the