Blue Baby Syndrome Lab Report

1935 Words8 Pages
Hali Ngobili Biol 2701k Lab Report Determining the Cause of Blue Baby Syndrome Due To High Levels of Nitrite in Drinking Water in Gopher Hollow Village Abstract: The CDC reported a cluster of blue baby syndrome in a village in which all families drew their water from the same well. The well water has a nitrate/nitrite level in excess of 50 ppm. There are four sites located along the rivers and stream upstream of the contaminated well that seems to be the main suspect. The sample sites include a brand new subdivision of high-priced “Mcmansions,”(Sample A) , a textile plant that produces Braves T-Shirt (Sample B), an organic farm with a herd of 50 cows and 10-acre field of zucchini (Sample C), and a mountain lake that has become overgrown…show more content…
To prepare the standard samples ten 16 x 100 mm glass test tubes are collected and 1 ml of the nitrite standard solutions was added at the following concentrations: distilled water, 0.5 ppm, 1.0 ppm, 2.0 ppm, 3.0 ppm, 4.0 ppm, 6.0 ppm, 10.0 ppm, 20.0 ppm, and 40.0 ppm. A micropipette tip is attached to a P20 micropipettor and the volume is set to 20 microliters (µl). 20 µl of the color indicator reagent (1-naphthylethylenediamine sulfanilamide) is drawn into the micropipette and transferred into each test tube with the nitrite standard solutions. The samples set for five minutes to allow the full color to develop before the OD₅₅₀ levels were measured. After the spectrophotometer light warmed up, the “A/T/C” (Transmittance/Absorbance/Concentration) control button was pressed to register Absorbance (A). The 1 ml of the nitrite standard solutions in the test tubes was transferred into cuvettes. The cuvette filled with 1 ml of distilled water was placed into the sample compartment. After the sample compartment lid was closed the “Blank” control button was pressed. The optical density (OD₅₅₀) registered on the screen at 0.000A. The cuvette was removed from the sample compartment and the liquid was transferred back into the test tube. The rest of the cuvettes with 1 ml of the nitrite standard solution of the different concentrations were placed in the sample compartment one at a time and the OD₅₅₀ levels were recorded. Next to measure the optical density of the sample sites four 16 x 100 mm glass test tubes were filled with 2 ml of water samples from the suspect sites. 40 µl of the sulfanilamide reagent was added to each test tube and the color was allowed to develop. 1 ml of the sample was transferred to the cuvette and the optical density was recorded. To dilute the required samples two 16 x 100 mm dilution tubes were labeled 1:10 and 1:50
Open Document