After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid. Place another dry test tube in a 50mL beaker and weigh it. Find a bottle of barium iodide and record the name and molar mass. Then, weight out either anhydrous barium iodide or barium iodide dehydrate into this test tube and dissolve is it in 2 mL of deionized water. Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form.
A cuvette block, four cuvettes, three 50 mL beakers, one 150 mL beaker, and one 5mL pipet were obtained. The phosphate buffers NaH₂PO₄ and Na₂HPO₄ were procured weighing .4060g and .4106g respectively. The phosphate buffers were then transported to the 150mL beaker. Next 50mL of distilled water was measured using a graduated cylinder and added to the 150mL beaker. Twenty drops of bromothymol blue was also added to the 150mL beaker.
* 3. 3.0 ppm standard: * 3.00 mL of 10.0 ppm phosphate solution was placed in a 25 mL graduated cylinder and diluted to exactly the 10 mL mark with distilled water then poured into a plastic cup labeled 3. Cylinder was rinsed with distilled water. * * 4. Zero standard: 10 mL of distilled water was poured into a plastic cup labeled 0.
Examine the effect of heat on the solubility of albumin B. Examine the effect of pH changes on the solubility of albumin and casein C. Examine the effects of 95% ethanol, lead(II) nitrate, silver nitrate, and tannic acid on albumin and casein Procedure A. The effect of heat Place about 1 mL of 2% albumin in a test tube and heat it in a hot water bath for a few minutes. Compare the appearance to the albumin solution at room temperature. B.
In the first part, five 100 mL flasks of 5 mL ligand solution, 5 mL 2 M sodium acetate, 4 mL 3 M NH2OH, and 1-5 mL Fe2+ solution are diluted with water. The absorption spectrum for varying concentrations of Fe2+ are measured using a spectrophotometer and the data is graphed in Excel. The slope of the line is ε in the Beer-Lambart equation A = εcl. In the second part of the experiment, eleven flasks containing diluted stock solutions of Fe2+ and ligand are mixed with 5 mL 2 M sodium acetate and 4 mL 3 M NH2OH and diluted with water. The absorption spectrum is measured using a spectrophotometer and the data is graphed in Excel.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
The two unknown solids are weighed to a mass of 0.15g each. The unknown solids are dropped carefully into the corresponding Erlenmeyer flask wit 50mL of distilled water. The solid in the water must be dissolved and afterwards add 10 drops of Bromecresol green to indicate the change of color when the solution has been titrated. The flask should start with a blue tint. HCl is carefully dropped into the Erlenmeyer flasks with the primed pipette until the solution turn to a green tint.
A) How many mosm solute will 1 gram of NaCl yield? Show your calculations. (1gNaCl/1)*(1000mg/1gNaCl)(2/58mg)=34.5mOsm. 3. Mixed Solutions: If 1 mmole of glucose (180mg=1mOsm) and 1 mmole of NaCl (58mg=2mmOsm) are put into a beaker and distilled water added to make 1 liter, the osmolarity is 3 mOSm/L.
Approximately 20 drops of a .04% Bromothymol blue solution was then added to the beaker of the phosphate buffer. Using a clean 5 mL serological pipette, transfer 5 mL of the phosphate buffer and Bromothymol blue solution to each of three clean 150 mL beakers. Next, using the buret for HCl, add 1 mL of HCl to one of the three beakers, then label this beaker “Yellow”. Next use the buret for NaOH and add 1 mL of NaOH to another of the three beakers, and label this beaker “Blue”. Lastly add 1 mL of water using the buret for water to the last beaker, and label this beaker “Green”.
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).