Problem: What is the effect of pH on beef liver catalase activity when it is measured by the change in temperature? The pH will be measured using a pH probe; and the temperatures will be measured using four temperature probes. Hypothesis: It is hypothesized that if the pH of the beef liver catalase solution is adjusted from its initial pH with the use of an acid or base, then a temperature difference will occur within the beef liver catalase solution. This is predicted because pervious experiments have shown that an increase in temperature may also lead to an increase in the number of ions in solution due to the dissociation of molecules. Since pH is a measure of the hydrogen ion
Effect of Surface Area to Volume Ratio on Diffusion Rate of Glucose from Potatoes Abstract: The experiment was conducted to determine if differences in surface area had a significant effect on diffusion rate of glucose from potatoes. Potato samples of three different lengths were collected through a process of coring along the middle of the vegetable. Cores were soaked in distilled water for a period before an o-dianisidine-enzyme solution was added. Glucose diffusion rates were determined by using a spectrometer after a period of incubation of the potato samples. Glucose levels were measured at 0.52, 0.57, and 0.67 showing that an increase in surface area corresponded with a non-significant (p=0.096) increase in the glucose diffusion rate.
In intact cells succinate loses hydrogen ions and electrons to FAD to form fumarate. This step in the TCA cycle will be used to study the rate of cellular respiration under different conditions. (Patriquin, M. Rand, T. 2012). Since DPIP is a reducing dye it will absorb the hydrogen ions and electrons from the redox reaction of the TCA cycle between succinate and fumarate producing a discoloration of the dye. The discoloration is measured in percent transmittance of light over 30minutes at 5 minute intervals.
When the fuels combust, oxygen and fuels react, and heat released. The water then absorbed the heat from the combustion. In this experiment the calorimeter of energy is used to measure the temperature of the heat that is released from the cashew. The equation to find the energy produce during
Record your hypothesis on page 123. -My hypothesis is we can determine the different between each chemical reaction by the temperature change.To descide which one is exothermic and endothermic. 4. Summarize the procedures you will follow to test your hypothesis. -We measured in each chemical and added water besides, HCl we added NaOH.
One way we can make renewable resources is by the alcoholic fermentation of ethanol. Alcoholic fermentation is the process where yeast breaks down sugars into 2 pyruvic acid, and carbon dioxide and alcohol, or in this case ethanol. Ethanol is a main product in fuel, and it is a by-product from corn. Research Problem: In this experiment, we’re going to measure alcoholic fermentation by measuring the carbon dioxide bubbles that are produced per minute after forty minutes. Hypothesis: I believe that we should be able to get multiple bubbles of carbon dioxide, because the carbon dioxide is produced producing small air sacs.
ABSTRACT The question to be asked in this laboratory experiment is if the rate of anaerobic respiration, alcoholic fermentation, is affected by a change in temperature. This question can be further researched by drawing the hypothesis that if temperature is above or below room temperature there will be an affect on this process of alcoholic fermentation. Overall, the purpose of this experiment is to test the hypothesis to see if varying temperature affects the rate of alcoholic fermentation or anaerobic respiration. The experimental design is an overall basic setup; there is a mixture of yeast, corn syrup, and water that is of equal ratio in all three fermentation tubes. After covering the open ends to the fermentation tubes and the entire mixture flows into the measuring portion of the device one tube is put in either an ice bath, boiling water, or stays at room temperature.
My group’s hypothesis is if that if we raise the temperature of the enzyme and the potato extract then the reaction rates will be considerably higher and more light will be absorbed when measured. The heating variable will denature the protein. In the experiment, I raised the temperature of the Catechol and the enzyme from room temperature to 70 degrees Celsius separately. I then combined the heated extract with the catechol and measured the amount of light that was absorbed by the product, benzoquinone. I timed it in 20 second increments and noted the result in a spreadsheet.
Isolation of Caffeine Introduction Caffeine is a stimulant found in the cacao plant that is used, today to increase alertness when taken in moderate doses. In this lab, we extracted caffeine from 2 NoDoz tablets, which contains 45% caffeine. We accomplished this task by performing a hot gravity filtration to separate the insoluble binder from the caffeine, a liquid extraction to separate the organic caffeine-containing layer from the aqueous layer, a gravity filtration to filter off the hydrated salts, a steam bath evaporation to evaporate out the organic solvent, and a recrystallization to obtain dry caffeine crystals. The ultimate goal of this lab was to acquire the same amount of caffeine as listed on the NoDoz tablets. Materials and Methods Materials The compounds used in this lab are listed in the table below: Procedure We boiled 2 NoDoz tablets in grinded form with 60 mL of water in a 100 mL beaker and allowed it to cool to room temperature after it had been sitting on the hot plate for 5-10 minutes.
Then measure the distance of each pigment starting at the bottom of the pigment origin to the bottom of the separated pigment band. In Exercise 4B: Photosynthesis/ The Light Reaction, chloroplasts are extracted from spinach leaves and incubated with DPIP in the presence of light. The spectrophotometer should be warmed up and set the wavelength to 605 nm. An incubation area should be set up, that includes a light, water flask, and test tube rack. We were provided with two beakers, one containing a solution of boiled chloroplasts and