2. What is the purpose of fixing a slide that is to be stained? The fixing slide kills cells quickly by denaturing enzymes, adhere cells to slide better to prevent wash off. 3. Why are the specimens to be stained suspended in sterile saline or distilled water?
ENZYME worksheet 1. Label the diagram [pic] a. substrate b. active site c. enzyme d. products 2. Answer true of false to the following statements: ___T____ Enzymes interact with specific substrates ___F____ Enzymes change shape after a reaction occurs ___T____ Enzymes speed up reactions. ___F____ One enzyme can be used for many different types of chemical reactions. ___T____ Enzyme reactions can be slowed or halted using inhibitors.
Explain what it means to denature an enzyme. -To denature an enzyme means to change its shape by breaking the hydrogen bonds that holds its shape, thus rendering it useless. An enzyme can be denatured with heat, or with a change in pH. 5. What factors did you need to control in your tests?
Denatured sucrase was used as a control because denatured enzymes, in this case sucrase, lose their shape and can potentially lose their ability to catalyze a chemical reaction. Using deantured sucrase as a control helps to further solidify and/or compare findings from solutions
| | | C) | Osmotic pressure caused the cell to rupture. | | | D) | The cell was damaged by bacteria. | | | | | | Feedback: Since the cells are in a hypotonic solution, water entered by osmosis until the osmotic pressure ruptured the cell. | | 6 CORRECT | | The semifluid environment inside the plasma membrane is called ____. | | | A) | endoplastic reticulum | | | B) | microtubules | | | C) | cytoplasm | | | D) | mitochondria | | | | | | Feedback: The cell's chemical processes take place in a semifluid material called the cytoplasm.
The breakdown of the membranes of these structures, affect the function of his heart cells because lysosomal enzymes which are normally bound safely inside vesicles will digest the plasma membranes and the membranes of the organelles. D.) Predisposition means that Joseph inherited vascular disease. Dna is in the nucleus of the cell. We need DNA to replicate or repair our cells. Also, cytosol is the site of chemical reactions needed to maintain cell structures and allow cell growth.
This allows| | |the release of other cells into the tissue |plasma and neutrophils to move from the blood vessels into | | | |damaged tissue | |4th |As the wounded part of the body reacts to the germs, |Polymorphs move to the bacteria by the chemotaxis and | | |certain cells destroy these germs |ingested. That destroys the bacteria and
1. Infectious agent. This is the disease-causing organism. For many illnesses and diseases this is a virus or bacterium. In order to break this link, various methods can be used, including the pasteurization of milk, the chlorination of drinking water, or the use of disinfectants.
c) Name 2 different methods of measuring pH of substances (in a laboratory)? • Probe and meter • Litmus paper 5. Explain the relationship between the natural pH of the skin and the action of: a) Microflora • The bacterial microflora on our skin are able to survive acidic conditions with a pH range 4-6. Our acidic skin protects us from harmful bacteria or pathogens, our microflora are able to breakdown the fatty acid molecules and thereby increase its
In Somatic gene therapy inserted genetic material to the target cells, does not pass on the change to the next generation. In comparison the opposite is true for Germ line gene therapy. Due to this evident difference, current regulations allows gene therapy only on somatic cells.1 There are two lines of treatment applications for gene therapy, one for the treatment of genetic diseases and the other for non-genetic and polygenic disorders.3 Two different approaches to administer gene therapy to patients exist. “In vivo” which involves a therapeutic gene being administered directly via a vector into the tissue of the patient. This vector interacts with the target protein to produce the required therapeutic protein.