3. Why is it necessary to make pure subcultures of organisms grown from clinical specimens? So that the organism can be identified and tested for antibiotic sensitivities. 4. What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
| | | C) | Osmotic pressure caused the cell to rupture. | | | D) | The cell was damaged by bacteria. | | | | | | Feedback: Since the cells are in a hypotonic solution, water entered by osmosis until the osmotic pressure ruptured the cell. | | 6 CORRECT | | The semifluid environment inside the plasma membrane is called ____. | | | A) | endoplastic reticulum | | | B) | microtubules | | | C) | cytoplasm | | | D) | mitochondria | | | | | | Feedback: The cell's chemical processes take place in a semifluid material called the cytoplasm.
6. Wash, dry and observe under oil immersion lens. Differential stains render one type of microbe one colour and other types of microbes another colour. In the Gram stain, Gram positive organisms retain the primary dye complex (crystal violet-iodine) whereas Gram negative cells loose the primary dye complex during the challenge rinse. Most differential stains have a challenge step that follows staining with a primary dye.
Investigation of the effect of Substrate concentration on Catalase activity Research Question: To investigate enzyme kinetics, using catalase enzyme from the yeast extract. Background Information: Enzymes are proteins which catalyze reactions that take place in the body or they increase the rate of the biological reactions. In an enzyme catalyzed reaction, the substrate binds to the active site and forms the enzyme-substrate complex with the enzyme. The enzyme breaks the bonds present in the substrate; the final product of this reaction leaves the enzyme which remains unchanged after the reaction. Catalase is a substance which is produced by the liver to break down hydrogen peroxide.
1-2-09 Glowing Bacteria Genes are pieces of DNA which hold the instructions for making proteins. The protein will then give an organism a particular trait. Genetic transformation means changes caused by genes, and requires the insertion of a gene into an organism, in order to change the trait. this technique is used by in many areas of biotechnology. Agriculturally, plants can be genetically transformed to be resistant to pesticides, spoilage, and frost.
Alpha hemolysis 3. Streptococcus pneumoniae are Gram-positive, lancet-shaped cocci (elongated cocci with a ... sensitive to bacitracin than other beta hemolytic streptococci. 4. the swelling of the capsule surrounding a microorganism after reaction with an antibody; the basis of certain tests for identifying microorganisms; "pneumococcus quellung" 5. capsules provide protection from viruses. they can be slippery to defend themselves from being eaten. Protects the bacteria from phagocytosis allowing the bacteria to stay in the body 6. pure culture 7.
In today’s presentation we will cover information regarding the organism that causes botulism and its epidemiology. We will also talk about the history of the disease, how it is transmitted, and the clinical and necropsy signs observed. Finally, we will address prevention measures for botulism. Botulism is caused by the bacterium Clostridium botulinum. It is a gram positive, spore-forming, obligate anaerobic bacillus.
Digestive enzymes are hydrolytic enzymes. Their substances, or the molecules on which they act are organic food molecules which they breakdown by adding water to the molecular bonds, thus cleaving the bonds between the subunits or monomers. Digestive enzymes can function outside the body cells; their activity can be studied by test tubes (Marieb and Mitchell 2010). This experiment attempts to re-create the breakdown process that is normally done via digestion with Iodine as a vital component. It can be expected that once amylase reacts with the starch, maltose will then be broken down and less starch will be visible and more sugar will be apparent thus causing the solution mixed with iodine to become lighter and lighter.
Aim: To investigate on the activity of catalase. Introduction Catalase, an enzyme found in many different tissues, catalyses the breakdown of hydrogen peroxide into water and oxygen. 2H₂O₂ → 2H₂O + O₂ Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide. Minced potato provides a suitable source of catalase and the pH is varied in this experiment using citric acid-sodium phosphate buffer solutions at pH values of 4.4, 5.2, 6.5, 7.5 and 9.
How are these electron carriers reoxidized in anaerobic bacteria? How are these electron carriers reoxidized in aerobic bacteria? 3. (24 points) Aerobic eukaryotic organisms use the unique abilities of mitochondria to extract further energy from glucose (and other nutrients). a) The citric acid cycle completes the degradation of glucose.