Biochem 208.5.1-02-05 Essay

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Biochemistry Western Governors University Biochemistry Competency 208.5.1-02-05: DNA, RNA By Marianne Stone DNA Replication (slide 2) 1. Parent Strand 2. Daughter Strand 3. Direction Of Replication 4. Replication Fork 5. A- Adenine 6. T- Thymine 7. C-Cytosine 8. G-Guanine 9. 3’ (3 Prime) 10. 5’ (5 Prime) 11. Lagging strand 12. Leading strand 13. Replication occurs in the 5’ to 3’ direction 14. Helicase unzips the parent DNA at the replication fork ("DNA replication," n.d.) Role of DNA Ligase (slide 3) 1. DNA polymerase goes from 5’ to 3’ 2. RNA primase lays the beginning for DNA Primase to begin laying down the nucleobases: Adenine, Thymine, Cytosine and Guanine. 3. Okazaki fragment from RNA primase a segment of the lagging strand during replication. 4. DNA ligase goes over all the small Okazaki segments and binds them into a new strand of DNA. 5. DNA polymerase replaces the RNA primer and DNA ligase binds the segments. (Hank, 2013) mRNA Transcription (slide 4) 1. mRNA 2. DNA (Hank, 2013) mRNA Transcription (slide 5) 1. Polypeptide chain 2. tRNA 3. Next amino acid to join chain 4. Codons 5. Cysteine 6. Leucine 7. Leucine 8. Aspartic Acid 9. Isoleucine 10. Proline 11. Stop Codon (Hank, 2013) Death Cap Mushroom (slide 6) Eating death cap mushrooms can cause symptoms in a little as 6 hours and thirty hours. (Shiells, 2013) Symptoms range from gastrointestinal discomfort to death from liver and kidney failure. Amatoxin - α-amanitin is an enzyme found in the death cap mushroom. It inhibits the formation of mRNA in the liver. Without the mRNA protein production in the liver is compromised and causes cell death. The amatoxin can recirculate through the bloodstream and cause repeated damage to the liver and kidneys resulting in liver and kidney failure and death.(Shiells, 2013, para. 8) References DNA

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