Bio Lab Report

Name : MUHAMAD HANIF BIN KAMAIL
Class : M09T
Title : ESTIMATING GLUCOSE CONCENTRATION IN SOLUTION
Date : 15/10/2009

INTRODUCTION: Glucose (C6H12O6) is a monosaccharide reducing sugar. In this reaction, the glucose readily donates the electrons which are accepted by the permanganate causing it to change colour. The purple pink solution of potassium permanganate (MnO4-) is reduced to a colourless solution of manganese ions (Mn2+).
Colourless in solution
Purple pink in solution
MnO4- + 8H+ + 5e- Mn2+ + 4H2O

As a result of this reaction the glucose is oxidised. The time taken for the loss of colour from a standardised solution of permanganate is directly related to the concentration of glucose present in the solution.

RESEARCH QUESTION:
What is the relationship between glucose concentration and the rate of reaction of the reduction of potassium permanganate?

HYPOTHESIS:
The higher the concentration of glucose, the shorter time taken for the reduction of potassium permanganate, hence resulting in shorter time taken for the pink colour of potassium permanganate to decolourise. This is because the concentration of glucose molecules in glucose solution is high thus more electron are donated to the permanganate within a constant period.

VARIABLES: Units | Range | Independent Variables | The concentration of glucose solution. | % | 0% - 30% | Dependent Variables | Rate of reaction of the reduction of potassium permanganate. | s-1 | 0.0057s-1 - 0.0442-1 | Table of variables

METHOD FOR CONTROLLING VARIABLES: Controlled Variables | How to handle | Units | Possible effect(s) on result | Volume of the indicator (potassium permanganate). | The volume of potassium permanganate is measured 2cm3 using syringe constantly. | cm3 | If the amount of potassium permanganate is excessive, the time taken for the reduction to complete may be