Bio Lab Essay

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How much light is absorbed by chlorophyll and what pigments are in this chlorophyll? Mohammad Sharif AP Bio Introduction In this lab there are two experiments, one which is the paper chromatography and the other is testing the amount of light which is absorbed by the chlorophyll. In the first experiment we will use spinach chlorophyll and separate its pigments using chromatography paper and ethyl alcohol or ethanol. After a while the ethanol will travel up the chromatography paper and separate the pigments in the chlorophyll solutions. In the other experiment we will test how much of light does the spinach chlorophyll absorbs. The control of this experiment will be the buffer solution; this solution will transmit 100% of light. Then we will test the amount of light absorbed by the chlorophyll solutions on carious wavelengths starting from the wavelength of 400 to as much as 700. Paper chromatography is a useful technique for separating and identifying pigment and other molecules from cell extracts that contain a complex mixture of molecules. The solvent (ethanol) moves up the paper by capillary action, which occurs as a result of the attraction of solvent molecules to the paper and the attraction of the solvent (ethanol) molecules to one another. As the solvent (ethanol) moves up the paper, it carries along any substances dissolved in it. The pigments are carried along at different rates because they are not equally soluble in the solvent (ethanol) and because they are attracted, to different degrees, to the fibers of the paper through the formation of intermolecular bonds, such as hydrogen bonds. Thus, separating the different pigments and the pigments would eventually be shown as the solvent

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