The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
What kinds of clinical specimens may yield a mixed flora in bacterial cultures? Oral, Skin, or GI specimens 5. When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination? Individual colonies can be picked up on the inoculating loop, or straight wire and inoculated in to the fresh agar or brother media References Cowan, M. K. (2012). MICROBIOLOGY: A SYSTEMS APPROACH, THIRD EDITION.
The tests performed and what constitutes a positive and negative test are as follows. 2. Test Result of all organism similar to my unknown See Following pages 3. Flow chart See page with flow chart 4. Test Result Oxidase This laboratory test is based on detecting the production of the enzyme cytochrome oxidase by Gram-negative bacteria if no color change to purple or blue is evident at 30 seconds-1 minute then the result is positive.
www.new-harvest.org This website gave me information on meat alternatives. This website also informed me about the public health and the damage factory farms do to the environment. www.fooddialogues.com This website gave me more information about factory farming. It also showed me articles posted by people who have seen the farms first hand and images of the farms.
The total sample volume was made up to 13 μL by adding water. The reaction vial was placed on ice, and was added 2 μL each of 10x NTP labeling mixture, 10x transcription buffer, and T7 RNA polymerase. 1μL of protector RNase inhibitor was also added, and the contents in the vial were mixed gently and incubated for 2 hours at 37 degree Celsius. To remove the template DNA after transcription, 2 μL of DNase I was added and incubated for about 15 minutes at 37 degree Celsius. The reaction was stopped by adding 2 μL of 0.2 M EDTA at pH 8.
Ehrlichia bacteria live within the white blood cells of their hosts. Wild animals, pets, or humans can all be a host of the ehrlichiosis bacteria (Veterinary Information Network,
First I made a water bath by filling the 100 mL beaker with cool tap water. I then placed crushed ice in the 100 mL beaker so the water level was just below the top of the beaker. I sprinkled a little salt in the ice water and mixed it well. I then filled the test tube half full with distilled water and set the test tube in the 24 well plate. I inserted the digital thermometer into the test tube and took reading every 30 seconds until the readings remained constant.
Would we be healthier and safer or is that just a load of manure. In this essay, I’m going to explore the differences between corn-fed and grass-fed beef and why grass-fed cattle are the better choice. Back before World War II, all cattle fed on grass. In an article published by Craig Walsh he wrote that during the war, “Farmers were
1-2-09 Glowing Bacteria Genes are pieces of DNA which hold the instructions for making proteins. The protein will then give an organism a particular trait. Genetic transformation means changes caused by genes, and requires the insertion of a gene into an organism, in order to change the trait. this technique is used by in many areas of biotechnology. Agriculturally, plants can be genetically transformed to be resistant to pesticides, spoilage, and frost.
Bovine Spongiform Encephalopathy (BSE) • BSE or bovine spongiform encephalopathy is a neurological disease of cattle. • It is believed to be transmitted to cattle from ground up meat and bone that had been infected from sheep products that were fed to the cattle. • The outbreak grew as young calves were fed meat and bone meal that was infected with the prion disease. • In order for the next