What kinds of clinical specimens may yield a mixed flora in bacterial cultures? Oral, Skin, or GI specimens 5. When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination? Individual colonies can be picked up on the inoculating loop, or straight wire and inoculated in to the fresh agar or brother media References Cowan, M. K. (2012). MICROBIOLOGY: A SYSTEMS APPROACH, THIRD EDITION.
Page 5 Basic Research (TCP, Dettol and E.coli bacteria) ……………………………… Page 5-7 Preparing my experiment ……………………………………………………………….. Page 8 TASK 3: Materials and Methods____________ TASK 3: Materials and Methods____________ Apparatus …………………………………………………………………………………………..
Unknown Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 Due Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae isolated from the given #103 was a Gram-negative Rod. The purpose of this lab was to isolate and identify the genus and species of an unknown bacterium. It is important to identify an unknown microorganism because knowing how the bacteria work and how it is structured means knowing how it can affect humans. Unknown bacteria may also be used clinically many pharmaceutical drugs are based on products made by organisms (Katzung, B.G.2004). In order to identify the unknown organism a series of tests were performed.
Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis. Materials and Methods: Materials Streptomyces and Antibiosis The following materials and apparatus were used to complete the study of Streptomyces and its antibiotic properties. Soil samples were collected from the pond on the Brooklyn College campus,
3. Plasmid pBR607 is a 2.6 kb plasmid containing Ampicillin and Tetracycline resistance markers, an origin of replication, and unique restriction sites for the restriction enzymes EcoRI, BamHI, and PstI. Given the restriction map for pBR607 for the enzymes EcoRI, BamHI, and PstI, show on the agarose gel picture below where the approximate positions of the restriction fragments generated from the given restriction digests would be located after carrying out electrophoresis. 4. As part of an undergraduate project, a student was attempting to construct a restriction map for the plasmid pUC23 using the restriction enzymes EcoRI and BamHI.
The equations we used to calculate the frequency and concentration of the gel was: number of colonies/dilution factor = concentration cells μl. Frequency = number of bacteria/total number of bacteria.
Flagellum is a crucial characteristic to the motility and colonization of Proteus mirabilis.it is also linked to the ability of this bacteria to form biofilms, assisting in the bacteria’s resistance to the host defenses and selecting antibiotics .It also acts as a surface sensor. P. mirabilis is a peritrichious flagellated bacterium .which gave it the ability for swarming motility. ( Armbruster and Mobely.2012; Belas and Suvanasuthi.2005). (32,124) These flagella form a bundle that propels the cells forward on the surrounding fluid(Tuson et al.,2013)(128) Proteus mirabilis elevates its flagellar number, from fewer than (10 to up to 5,000), and its length from (1 to 2 m )to (20 to 80 m), over differentiation from the swimmer to the swarmer cell type (McCarter,2010)(126) Adhesins fimbriae 1.4.1.2- Fimbriae are appendages on the bacterial surface which is used for adherence (Morgenstein.2011) (129), consisting of a polymer of a single polypeptide subunit interspersed with adhesive proteins. The fimbriae are arranged around the bacterial cell in a peritrichous form (Rocha et al.,2007)(130).
September17.2013 P.4 Environmental Sources Of Bacteria Purpose: We did this lab to find out exactly which conditions would help to grow a bacterium the best. Materials: • Agar • Petri dish • Cotton • Incubator • Tape • Bacteria Procedure: 1. Mark bottom of Petri dish 2. Split petri dish into 4 sections and label the sections 3. Pour hot agar into dish 4.
02 April 15, 2014 #7 Enterobacteriaceae, Escherichia coli Staphylococcaceae, Staphylococcus epidermidis ABSTRACT The objective of the Unknown Report is to identify two unknown bacterium based on a series of tests learned in the microbiology laboratory. The tests performed on organism A were Fluid Thioglycollate, Gram Stain, MR/VP, SIM, Simon Citrate, and LB plate. The results for organism A were Facultative Anaerobe for aerotolerance, Gram negative, MR positive and VP negative, the SIM test results were positive for Indole productivity and growth radiating but negative for sulfur production, Simon Citrate negative, and there was no red growth on the LB plate. These tests lead to the discovery of the bacterium Escherichia coli which
W5_A2 Microbiology Lab Week 5 Review Sheet I Exercise 1: Hanging-drop and wet-mount preparations 1. How does true motility differ from Brownian movement? Brownian movement results from random motion of water molecules bombarding the bacteria and causing them to move. True motility and self- propulsion involves the 3 modes of motility. 2.