Bacterial Inhibitor Lab Report

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Mary Riddell 9/26/11 Period 3 The Zone of Inhibition Created by an Antiseptic, Disinfectant, or an Antibiotic Abstract: The purpose for conducing this experiment was to determine which bacterial inhibitor would create the largest zone of inhibition on bacteria streaked petri dish. In the experiment it was made clear that the antibiotic had the largest zone of inhibition, the antiseptics had the second largest zone of inhibition, and the disinfectant had the smallest zone of inhibition. The antibiotic worked the best because antibiotics work on the inside of the cell to break them down while the others are meant to sit on top of the surface to kill them. Introduction: The purpose for conducting this test was to determine which bacterial inhibitor would have the largest zone of inhibition on a bacteria streaked perti dish. The bacterial inhibitors in the experiment that were chosen were, an antiseptic, (hydrogen peroxide), a disinfectant (ammonia), and an antibiotic (tetracycline). The hypothesis stated that the ammonia with have the largest zone of inhibition because it is a strong disinfectant and will kill most of the bacteria. A disinfectant is made for killing bacteria on surfaces. Materials and Methods: To start the experiment swab bacteria from and existing petri dish. Next, take the cotton swab and drag it lightly in a zigzag motion across the agar on the petri dish; this is called streaking. Create two sections in each of the petri dishes by drawing a line down the middle of the outside plastic with a permanent marker. Then, place the four bacterial inhibitors disks in the center of the four sections of the petri dish. Make sure to soak each disk in the bacterial inhibitor for twenty-four hours. Lastly, put the lids back on the petri dishes and put in the incubator at 37 degrees Celsius for 24 hours. Measure the bacterial

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