Bacterial Genetics and Genetic Transformation Essay

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BI108 Lab 7 Bacterial Genetics and Genetic Transformation Study of Genetic Transformation in pGLO Abstract This experiment examines the bacterial transformation. We study the transformation by taking the genes from one organism and infusing them into another organism. A green fluorescent protein found mainly in the bioluminescent jellyfish Aequorea Victoria was extracted and used in the experiment. This specific trait – glowing under UV light – will be an observable trait when the gene infused with the organism. The transformation process involved the aid of a plasmid, a small circular piece of DNA. For this experiment, a pGLO plasmid was used. This plasmid has three unique genes which is the GFP, an antibiotic resistance gene that results in the production of the enzyme beta-lactamase and a gene regulation system (M Chalfie, 1994). In the 21st century, genetic transformation is no longer foreign and was explored in many areas especially in biotechnology industry. The possibility of inserting a gene into an organism in order to change the organism’s trait is an interesting discovery. It opens up pathway to future research as scientists can now pinpoint the good genes and isolate the bad genes. Besides in biotechnology, genetic transformation has been seen in the medical field where gene therapy was utilised to insert healthy copies of the defective genes into a patient’s cells. In addition, this transformation has been more commercialised in recent years as researchers have managed to use this transformation to make genetically modified crops which is a product of extensive gene coding for favourable growing traits. Introduction In this experiment, we utilised a strain of E.Coli bacteria. The original, transformed E.Coli bacteria do not possess the antibiotic resistance gene. However, we transformed a strain of E.Coli bacteria with plasmid DNA. This

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