It is a gram positive, spore-forming, obligate anaerobic bacillus. The clostridial spores are ubiquitous in soil and are very resistant to heat, light, drying and radiation. Spores may survive boiling for several hours at 100 oC, however exposure to moist heat at 120 oC for 30 minutes will kill the spores. Specific conditions are required for the germination of spores. These include anaerobic conditions (such as rotting carcasses or canned food), warmth, and mild alkalinity.
· Broth culture of E. coli · Tubes of sterile nutrient broth · Inoculating loop · Bunsen burner · disinfectant Procedure 1. Disinfect the work surface by spraying it down with a disinfectant and then wipe with paper towel 2. Light Bunsen burner 3. Sterilize inoculating loop by holding it over the flame of the Bunsen burner until the tip becomes bright red. 4.
The buffer solution is used to keep the pH of the experiment at a constant number while carrying ions that are essential to hold a current. Agarose is a polysaccharide usually extracted from algae. It comes in a powder but when mixed with boiling water and poured into the chamber it transforms into a gel. A comb is put into place before the Agarose is poured to allow wells where samples will be deposited when the Agarose hardens. When Agarose is in a gel state it is filled with tiny pores that allow molecules to pass through.
b) Open the cover of the petri dish halfway and pour in the agar to just cover the bottom of the dish. Try to minimize the introduction of bubbles. c) Repeat for all the dishes. d) Immediately rinse the flask with warm water to facilitate washing the flask. 5.
This kind of Staphylococcus aureus is called “MRSA”, referred to methicillin-resistant S. aureus. This bacteria are multi-resistant to beta-lactam antibiotics, meaning that they are resistant to different kinds of antibiotics, such as penicillin, methicillin, dicloxacillin and cephalosporin. As the process goes, that normal Staphylococcus aureus are being killed by penicillin antibiotic, and the ones that are resistant survived and keep on reproducing, Staphylococcus aureus bacteria that is penicillin resistant spread in the community and its amount kept on increasing. This made natural selection to occur, which the bacteria that are antibiotic resistance are suitable ones and they survived in
Control Test Various material used in the previous tests were also tested as controls. All samples collected in the Petri dishes from the three tests were incubated for several days. In accordance with common sense, the samples collected from the unwashed fingers and benches will exhibit the largest growth of microbial colonies, while the samples collected after the fingers and benches were washed with either soap or disinfectant will exhibit a lower growth. As for the time test, the Petri dishes exposed the longest to the open air will see
The Effects of Physical Sound to the Growth-Production of Bacteria INTRODUCTION Background of the Study Microbiologists often have difficulties in culturing different bacteria (Watson et al., 1993). It takes too much time for them to culture some bacteria. Microorganisms have the ability to communicate with each other via chemical signals which has been the focus of the most investigations and physical signals which has received only limited attention (Reguera, 2011). Physical signals increases the oscillatory motions and polarization of the cells that lead to the production of sound waves, electromagnetic radiation or electromagnetic signals. Carbon and graphite can be a big help in enhancing the growth of the bacteria and convert external electromagnetic energy into sound (Matsuhashi et al., 1998).
Biology Lab Report Bacterial Colonies in Your Environment Purpose The purpose of this lab is to culture, examine and describe microorganisms (bacterial colonies) from sources in daily surroundings that we are exposed to. Variables and Controls The independent variable was the places we decided to swab for bacteria. The dependent variable was the types of bacteria that grew within the agar plate The controlled variables were the temperature in the sir, how tight the agar plate was sealed and how much food was in the agar plate. Materials -a sterilized agar plate (with lid) -markers -soap and water -clear tape -sterile cotton swabs Lab Safety In this lab we needed to wash our hands constantly before and after the lab. We were dealing with bacteria that has the ability to be harmful and to avoid illness/sickness we need to thoroughly wash our hands and be careful around the bacteria.
6. Wash, dry and observe under oil immersion lens. Differential stains render one type of microbe one colour and other types of microbes another colour. In the Gram stain, Gram positive organisms retain the primary dye complex (crystal violet-iodine) whereas Gram negative cells loose the primary dye complex during the challenge rinse. Most differential stains have a challenge step that follows staining with a primary dye.
| Practical Report | | | | Practical Report Introduction This specific experiment will demonstrate both the efficiency and the disinfecting capabilities of the various disinfectants used and if the experiment is followed correctly the results deemed should be noted that if the substance possess a chemical substances that inhibits the growth of the bacterial culture placed onto the agar (E.Coli) that inhibition zones will be created and we can tabulate and record the observation thus walking away with an understanding of the efficiency of the tested disinfectants used and their success rate. What is known about E.Coli is that it is normally found in food it is a Gram negative, rod shaped bacterium that can help the body break down and assists the body in digestion of food (Dowshen, 2009).Although certain strains of the E.Coli are pathogenic to humans such as the serotype O157:H7, this specific strain of E.Coli is known to cause food poising and can escalate and become life threatening (Nordqvist, 2011). Domestos is chlorine based bleaching agent (Directa, 2009) and is a common household cleaning substance that is used. Handy Andy is a common and popular general house hold cleaner. Dettol is trusted antiseptic and disinfectant and is widely used to fight unwanted bacteria (Thompson D. M.) It has a distinct and characteristic phenolic odour.