Obtain beaker filled with 250 mL of HCl and 5 pennies. 2. Get a pipette and remove 10 mL of that solution (HCl + Zn) and place it in a 100 mL beaker. 3. Add color indicator 4.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Then measure the final mass of the fuel using a mass scale. Record all the results. Repeat all the steps again, but change fuel (Cheetos, half a cashew nut, wax candle and ethanol) every three trials. Data Collection and Processing: Fuel | Trials | Mass H2O (ml) ±1 | Change in Water Temperature (°C) | Mass Loss of Fuel (g) ±0.02 | Energy Produced (J) | Energy Produced per grams (J/g) | Cashew | T1 | 200 | 5 | 0.31 | 4180 | 13483.87
Poinsettia 1. Inflate a red 260 all the way, then slowly release the air to let the whole balloon get very soft. As soon as the nipple end begins to shorten, stop releasing air and tie the nozzle to the end of the nipple. Find the exact middle and hold the two parts parallel. Roll one of the bubbles to make a twist between them, giving you a full-sized, soft double bubble.
NCSU – Dept. of Chemistry – Lecture Demonstrations Gas Law / IMF Common Gas Law Experiments Collapsing Balloon (Charles’ Law) Description: A filled balloon shrinks when immersed in liquid nitrogen. Materials: Liquid Nitrogen Dewar Insulated Gloves Procedure: Carefully fill dewar with N2 (l). Add balloons to the dewar (suitcase analogy often used). Remove balloons with tongs to show the decrease in volume of the gas inside.
Twenty drops of bromothymol blue was also added to the 150mL beaker. The pH was then obtained using the Vernier pH probe and it read 6.68. The 5mL pipet was then used to transfer 5mL of the green solution to the three 50 mL beakers. A 100mL beaker was obtained and filled with 1.0 M HCl solution and the pipet was used to acquire 1mL of the 1.0 M HCl solution. The 1mL of HCl was then transferred to one of the 50mL beakers turning the color of the solution to yellow.
* 3. 3.0 ppm standard: * 3.00 mL of 10.0 ppm phosphate solution was placed in a 25 mL graduated cylinder and diluted to exactly the 10 mL mark with distilled water then poured into a plastic cup labeled 3. Cylinder was rinsed with distilled water. * * 4. Zero standard: 10 mL of distilled water was poured into a plastic cup labeled 0.
Materials and Methods Part 1 – Cation Tests Potassium, iron (III), zinc (II), copper (II), and cobalt (II) cation solutions were made subject to two elimination tests involving the addition of sodium hydroxide in one and ammonium hydroxide in the other. Approximately 10 drops of each cation solution were placed in 10 different centrifuge tubes. To begin the sodium hydroxide test, 6M NaOH solution was added to one sample of each cation solution until either a precipitate was formed or until 20 drops were added. An additional 10 drops of 6M NaOH was added to each solution in which a precipitate formed and the solutions were shaken lightly to aid in the mixing of the reaction. To begin the ammonium hydroxide test, 15M NH4OH was added to one sample of each cation solution until the formation of a precipitate was observed, with care not to exceed 20 drops.
Put exactly 5.0 mL of water in the 10.0 mL graduated cylinder. Record this volume in your data table (10.0 mL). Label the first pipet "Acid." To calibrate the pipet, fill it with LIU-2 water. Holding the pipet vertically, add 20 drops of water to the cylinder.
The supernatant was transferred to a 1.5 ml tube; to which 225 µl of 5 M NH4Ac was added, mixed gently, incubated on ice for 30 min and centrifuged at 10,000 rpm for 15 min at 4°C. Then the supernatant was transferred to a fresh tube, 510 µl of isopropanol was added to precipitate the DNA and centrifuged immediately for 10 min at 10,000 rpm. The supernatant was removed; the pellet was washed twice with cold 70% ethanol, air-dried and suspended in 50 µl TE