Measure the solution by right clicking on the beaker and choose pH meter *Then I had to measure the pH of 0.1 M sodium hydroxide Get a 100 mL beaker from the equipment menu Right click on the beaker, select chemicals, and add 50 mL of 0.1 M sodium hydroxide. Measure the solution by right clicking on the beaker and choose pH meter Part 2: *First add 35 ml of unknown acid a to 100 mL beaker. Select all chemicals from the toolbar in the chemicals section, choose unknown acid a. Put the volume at 35 mL in a new 100 mL beaker. *Then add two drops of phenolphthalein indicator to the beaker by right clicking, choosing indications, and adding 2 drops of phenolphthalein.
Using a measuring cylinder, add 50cm3 of 1.0mol dm-3 sulphuric(VI) acid to the thyme extract in the conical flask. 8. Titrate the solution in the conical flask with the potassium manganate(VII) solution until a pale pink colour persists for 10 seconds. 9. Repeat the titration until there are two titres within 0.1cm3 of each other.
Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base, NaOH, helps bring the pH of the acid, HCl, closer to seven, which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration formula, Nb+Ma+Va=Na+MbVb , the molarity is able to be determined.
* The sodium carbonate solution was poured into the beaker with the calcium chloride solution. * A sheet of filter paper was folded in half twice and weighed. (1.1g)/(0.7g) * The filter paper was placed in a funnel in a paper cup. * The contents of the beaker were swirled to dislodge any participate from the sides and then slowly poured through the funnel. * 5ml of distilled water was poured down the sides of the beaker, swirled and poured into the funnel.
ISOLATION OF EUGENOL FROM CLOVES BY DESTILLATION + PUPOSE To perform distillation using a distillation apparatus and isolate eugenol from cloves. + EXPERIMENTAL The apparatus shown in figure was assembled using a 50-mL round bottom flask as the distillation pot. The distillation pot was charged with 2 g of ground cloves and 25 mL of distilled water. The cloves were allowed to soak in the water until thoroughly wetted (about 10 min), then the mixture was distilled, the distillate being collected at the rate of about one drop every 2 – 3 seconds. After about 10 mL of distillate were collected, the distillate was extracted with 5.0 mL of CH2Cl2 (aka DCM), then again with 5,0 mL of DCM.
For the first solution, pour 5 grams of sugar into 250ml graduated cylinder and add water up to the 250ml mark. Place the plastic covering on the top of the cylinder and mix the contents. When finished, place the contents into the sauce pan. Fill up the cylinder, again, with 250ml of water and place into the same sauce pan. Heat the mixture
Procedure Preparing of Medium Agar Materials and apparatus * Distilled water * Nutrient agar powder * Autoclave machine * Petri dish * Hot plated * Conical flask Procedure 1. 500 ml of agar solution was prepared by using 500 ml distilled water and 10 g of nutrient agar powder. 2. Both distilled water and nutrient agar powder was poured into conical flask. 3.
Add 0.5 ml concentrated HCl and 1.0 ml 15% KI solution. Mixed exactly 1 minute and leave for 5 minutes in a dark place. Add 0.5 ml starch solution, 20 ml distilled water. Mix and titrate with sodium thiosufate solution. Calculate the exact normality of Na2S2O3 knowing that in this chemical reaction 1 gram-equivalent of K2Cr2O7 react with 1 gram-equivalent of Na2S2O3 (1 mole K2Cr2O7 react with 6 moles Na2S2O3).
DNA Extraction Aim: To extract DNA from a carrot using house hold products. Method: * Step 1 – Pour 60 ml (¼ cup) of clear alcohol into a glass. Place the glass into the bowl of iced water to chill, then set aside. * Step 2 – Pour 120 ml (½ cup) of tap/distilled water into the measuring cup with the salt. Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved.
Oil-in-Water & Water-in-Oil Emulsions Introduction In this activity we will compare water in oil emulsions and oil in water emulsions with water-soluble food coloring. Material 5 small cups Microscope slides Food coloring 5 coffee stirrers Whole milk Butter Margarine Mayonnaise Vinaigrette dressing To Do and Notice Bring butter and margarine to room temperature before the start of the activity or melt 1 tablespoon of each in the microwave for 30 seconds. Measure out approximately one tablespoon of butter, whole milk, margarine, mayonnaise, and salad dressing into 5 separate small cups. With a dropper place a few drops of food coloring onto the surface of each sample. Mix each sample with a coffee stirrer then use the stirrer to spread a thin layer of each stained sample on a microscope slide.