Analysis of Escherichia Coli Growth to Gauge Efficacy of Antibacterial Mouthwash and Aseptic Technique

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Analysis of Escherichia Coli Growth to Gauge Efficacy of Antibacterial Mouthwash and Aseptic Technique Introduction Bacteria are the oldest life form on earth. They are ubiquitous, highly adaptable and some even thrive in harsh environments. They inhabit other organisms, some in a supportive capacity and others in a pathogenic capacity. Most Escherichia coli strains are harmless and even beneficial, aiding in digestion, creating vitamin K₂, and inhibiting the colonization of pathogenic bacteria in the intestine. E. coli has even been used to synthesize human insulin. Other strains are pathogenic, causing diarrhea, urinary tract infection and even fatal illness. Unlike some bacteria, E. coli cannot tolerate harsh environments, and instead thrive at 37°C. The primary goal of this experiment is to examine the growth of E. coli on bacterial lawns in order to determine expired mouthwash’s efficiency as an antibacterial product. Since it is expired, the mouthwash should not perform well as an antibacterial agent. The variables in this experiment are three different dilutions of E. coli culture in a nutrient broth. There are 3 test plates (one for each dilution) and 3 control plates, which are not treated with mouthwash. This experiment is relevant because E. coli is bacteria that mouthwash should realistically be expected to treat and many people rely on mouthwash to treat E. coli and other bacteria which may propagate in the mouth. The secondary goal is to practice aseptic technique while producing bioaerosols. Bioaerosols are produced, in this experiment, by suspending vapor of liquid biological agents in the air by pipetting. Bioaerosols, including those from E. coli, were collected on an agar plate. The E. coli used in this experiment were a beneficial species but were treated as a virulent species. Aseptic technique was used in generating bacterial lawns

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