Analgesic Lab Report

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Extraction and Purification of Components in an Analgesic Tablet November 2, 2014 My signature indicates that this document represents my own work. Excluding shared data, the information, thoughts, and ideas are my own, except indicated in the references. 1 Abstract: HPLC is a very important technique used today in many pharmaceutical fields. It helps separate the solutions. After extraction was performed on the tablet it was then ran through an HPLC test. After performing HPLC and using the line equation three concentrations were found as well as the three main ingredients. The three main ingredients were caffeine, aspirin, and acetaminophen. The final concentrations were 0.02 mg/mL, 0.06 mg/mL, and 0.21 mg/mL. It…show more content…
It displays the peak area on the Y axis and the concentration on the X axis. 9 Conclusion: The main purpose of this lab was to find the main ingredients in an Excedrin tablet as well as how much of each ingredient is in the tablet. Two different techniques were used to help develop the ingredients. The two techniques used were extraction and HPLC. It was concluded that Excedrin tablets consist of acetaminophen, aspirin, and caffeine. Both of these techniques are both effective when needed to find the area and concentration. After using three different calibration curves and the line formula; final areas were found for each ingredient. Acetaminophen had a concentration of 0.02 mg/mL caffeine 0.06 mg/mL and aspirin 0.21 mg/mL. It is concluded that these are the three ingredients in a tablet of Excedrin. By using the line equation and the calculations represented in appendix A, this is how much of each ingredient is in an Excedrin tablet. References: 1. Williamson, Kenneth L., and Katherine M. Masters. Macroscale and Microscale Organic Experiments. 6th ed. Belmont, CA: Brooks/Cole, 2011. Print. 2.

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