Sucrase Activity Lab Report

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PREDICTIONS 1. Sucrase will have the greatest activity at: pH 3 2. Sucrase will have the greatest activity at: 70˚ C 3. Sucrase activity: increases with increasing sucrose concentration MATERIALS AND METHODS Effect of pH on Enzyme Activity 1. Dependent Variable: amount of product (glucose & fructose) produced 2. Independent Variable: pH 3. Controlled Variables: temperature, amount of substrate (sucrose) present, sucrose + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Dependent Variable: amount of product (glucose & fructose) produced 2. Independent Variable: temperature 3. Controlled Variables: pH, amount of substrate (sucrose) present, sucrose + sucrose incubation time Effect…show more content…
Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5? 3. State the optimum temperature for sucrase activity, and describe how sucrase activity changes at lower and higher temperatures. The optimum temperature for sucrase activity is 40˚ C. Sucrase activity drops at lower and higher temperatures (it kind of levels out with minimal change at higher temperatures but is still dropping). See Table 3: Effect of Sucrose Concentration on Sucrase Activity See Graph: Effect of Sucrose Concentration on Sucrase Activity 4. Was sucrase activity higher at 25 ºC or 55 ºC? 5. State how sucrase activity changes with increasing sucrose concentration. Sucrase activity steadily increases with increasing sucrose concentration until a plateau is reached. 6. Was the rate of increase of sucrase activity greater when sucrose concentration went from 2.5 to 7.5 g/l or when it went from 22.5 to 27.5 g/l? pH Along the GI Tract Organ pH Mouth 6.35 – 6.85 Stomach 1 - 3 Duodenum (first section of small intestine) 6 Jejuneum (second section of small intestine) 7.5 Ileum (third section of small intestine) 7.5 Colon 7.5 -

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