Allow a few drops of the EDTA solution to drip into the beaker. This fills the tip of the titrator and removes any air bubbles from the tip. Pour the few drops of EDTA solution from the beaker down a sink or toilet drain, and then flush with lots of water. Wash the beaker well with soap and water to remove all the EDTA solution. Well rinse the beaker, first with tap water and then with distilled water.
DNA Extraction Aim: To extract DNA from a carrot using house hold products. Method: * Step 1 – Pour 60 ml (¼ cup) of clear alcohol into a glass. Place the glass into the bowl of iced water to chill, then set aside. * Step 2 – Pour 120 ml (½ cup) of tap/distilled water into the measuring cup with the salt. Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved.
Next, I used a brush and soap to wash my hands for 2 minutes, and then touched the third section. Then I repeated the previous step, but instead of 2 minutes, I washed for 4 minutes. For the fifth section, I used hand sanitizer on my hands, and then
Obtain an Erlenmeyer flask that has a vacuum opening and attach the vacuum tube to it 8. Insert funnel and rinse the funnel with distilled water 9. Turn vacuum on and pour the pink solution into the Erlenmeyer flask, the precipitate should stay on top of the filter 10. Rinse the beaker out until all residue is gone 11. Try to rinse the precipitate in the funnel until the pink color is gone 12.
By ways of decanting (using a class stirring rod to guide solution), pour the solution into the funnel until it is about half an inch from the top. The solution should be clear and colorless 8) Allow the flask to cool. While the flask and solution are cooling, wash the funnel and beaker with LOTS of tap water to remove all traces of KOH. Also throw away any carbon on the filter paper. 9) Once the solution is cool enough to touch, add 35mL of 3M H2SO4 (measured out in 50mL graduated cylinder) SLOWLY AND STIR CONSTANLY.
Add two drops of food coloring to the beaker of distilled water and mix thoroughly. Measure out a dropperful of bleach into a plastic pipet and set it aside until Step 8. 6. Calibrate the colorimeter a. Prepare a blank by filling an empty cuvette ¾ full with distilled water.
Changing to a calendar year end has little useful effect, and it requires that Smithon produce a short-year tax return from Dec to Jan, which is a relatively unnecessary administrative expense. What potential income tax ramifications exist for Mr. Johnson personally if he purchases the stock of Smithon and converts it to an S corporation? A: Mr. Johnson will be personally taxed on all of Smithon's income. This has the advantage of eliminating the double taxation usually associated with a C-Corporation. However, it will increase Johnson's current tax liability.
Add 1 drop of Na2 EDTA to C2 and stir, add more drops until color changes, record observations 9. Suck up the mixture in C3 into a pipet 10. Place the C3 pipet in hot bath. Attach pipet to side of beaker with clothespin to prevent it from floating, 11. Repeat step 10 but with C4 and place in ice bath.
* Pour the solution into a dry, clean sep funnel. * Wash it with 1 ml deionized water. * Swirl the mixture, and allow the 2 layers to form and separate. * Add another 5 ml of 6M NaOH to the organic layer and repeat the extraction. * Place the Erlenmeyer flask containing the aqueous layer in ice bath and slowly add a minimum of 3.5 ml of 12M HCl.
Bring more water to boil and add the blanched peel to the boiling water for 5 more minutes. Remove from the heat and drain off the water. 4) Mix 2 spoonful of granulated sugar and 1 cup of water in the saucepan. simmer it while stirring it constantly until the sugar dissolves completely. Add the orange peel back into the saucepan, simmer the sugar mixture for 3 minutes.