The seaweed will be cut and weighed (6 grams) and transferred into 150 mL solution. Using 40 mL of distilled water the seaweed is heated just under boiling for five minutes. After cooling, a filter will be used to remove the seaweed from the extract. The goal is to transfer 2-3 mL of filtrate into the evaporating dish. We now slowly pour the solution into a funnel with filter paper.
| Higher | Higher | Lower | Higher . | Lower | Higher | 6 Distribution of an animal and a plant Quadrant No. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Total | Total area sampled | Average per metre | Seedlings | 1 | 3 | 3 | 2 | 15 | 23 | 39 | 35 | 35 | 9 | | | | Crab Holes | 0 | 14 | 20 | 22 | 10 | 0 | 1 | 2 | 0 | 11 | | | | Distance from creek (m) | 5 | 10 | 15 | 20 | 25 | 30 | 30 | 35 | 40 | 45 | | | | Part B – Interaction in the ecosystem and impact of human activity Interactions found in ecosystem: Producer | Herbivores | Carnivores | Decomposer | Mangrove tree | Crabs | Crabs | Bacteria | | Snails | Snails | | | Fish | Eel | | | | Fish | | | | Skinks | | | | Foxes | | 7 Human Interactions
After the 48 hours the eggs were removed, rinsed and examined. One of the shell-less eggs was then fully covered in water, the other in corn syrup for 24 hours. After 24 hours the eggs were removed and examined. Observations and Results: After being in the vinegar for
Then, wait at least ten minutes for the zinc to settle. If after ten minutes the solution is not clear remove the solution with a dropper being careful not to get any of the zinc. Obtain filter paper and funnel. Transfer the solution from the dropper drop wise into a new test tube through the filter paper. For every 20 drops of solution you will add 0.1g of zinc to the new test tube.
In June of 2000 the cap requirements were changed from a multi-media cap to a geosynthetic clay cap. OU2’s elements were ground water removal via on-site extraction wells, ground water treatment (air stripping) for removal of VOC’s, discharge to a local water treatment plant and continued monitoring. In 2005 an amended report eliminated the removal and treatment part and, instead, replaced it with an injection of vegetable oil as a bioremediation of the VOC’s with monitoring. OU’3 excavation and stabilization of contaminated soils, on-site disposal of the stabilized soil, installation of a low permeability cap over the treated soil, placement of one foot of topsoil over the remainder of the uncapped site and placement of controls. The clean up approach for OU4 was to allow the clean up of the other operable units to address the contamination of the wetlands.
Procedure 1. Begin to prepare an EDTA solution. Weigh out 3.62-3.64 g of NaH2EDTA and record exact mass. Add the weighed amount to a 250 ml volumetric funnel carefully using a funnel Wash the funnel with water to ensure all of the solid is delivered to the flask Add 100-200 ml of water and mix. Enough water should be added so that the flask is full to the 250 ml mark Tightly wrap the top of the flask with a Parafilm when finished 2.
Then the blower was turned on for sufficient duration and UV lights were switched on for one hour. LAF microbial contamination test (Settling plate method) A total of three petri dishes were prepared aseptically inside a laminar air flow (LAF), and then the petri dish was filled by pouring sterile Tryptone Soy Agar (TSA) liquid media and allowed to solidify. One test media was placed in the LAF cabinet, one test media is placed beside the LAF cabinet while the other the test media was placed near the door. The three petri dishes lid were opened and allowed to stand for 15 minutes and closed again. Then, the test media is then incubated at 37 ° C, for 18-24 hours.
The reaction with pH 7 was used as a control. Each tube contained 1 ml of potato extract, 6 ml of pH buffer, and 3 ml of catechol. Tubes were covered in parafilm and mixed thoroughly as each reagent was added. For each tube, catechol was added last to prevent the reaction from beginning prior to data collection, and each reaction was conducted in triplicate. To determine the amount of benzoquinone produced, a color chart corresponding to benzoquinone concentration (Figure 1) was used
Sac #1 placed into beaker #1 with distilled water, sac #2 placed into beaker #2 with 40% glucose solution and so forth. 3. Before analyzing, we had to allow sacs to remain undisturbed in the beaker for 1 hour. 4. After 1 hour we boiled a beaker of water on the hot plate (for Benedict’s and AgNO3 test).
4. Submerge the bag in the beaker and leave overnight. 5. Dry off the bag and then record its mass. After this write down the color of the bag and the beaker solutions and then test the bag and beaker solutions for the presence of glucose.