Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base, NaOH, helps bring the pH of the acid, HCl, closer to seven, which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration formula, Nb+Ma+Va=Na+MbVb , the molarity is able to be determined.
DNA Extraction Aim: To extract DNA from a carrot using house hold products. Method: * Step 1 – Pour 60 ml (¼ cup) of clear alcohol into a glass. Place the glass into the bowl of iced water to chill, then set aside. * Step 2 – Pour 120 ml (½ cup) of tap/distilled water into the measuring cup with the salt. Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved.
The system was heated for 4-5 hr under vacuum at 200°C and then cooled down to the temperature (50°C) where we want to perform the adsorption study. Small doses of test gases consecutively introduced to the system and gradually increased up to 50 Torr until an equilibrium pressure was reached. Then the obtained differential heats of the test gases adsorption were recorded as a function of its coverage. Further, the manifold degassed under vacuum for almost 30 minutes; adsorption was conducted in the same manner. Finally, the number and strength of active surface are obtained from the difference between the adsorbed gases from the first and second
Methlyene chloride was a good solvent in this case because its boiling point is 40 degrees C and will boil off before the trimyristin which has a boiling point of 56-57 degrees C. Finally acetone was added to wash the crystals and the solution was vacuum filtered. This process was repeated and the trimyristin was collected. Results: Experimental yield of trimyristin= .64g Experimental melting point of trimyristin= 59 degrees C Percentage yield = experimental-truex100 True Dicussion: The extraction of trimystrin left white crystals resembling a powder. Its melting point was 56 degrees C which was exactly the recorded value. However, given that
The last sample prepared was at 25ᵒC. When mixing the solutions, the CaCl2 was slowly added to the NaCO3 at a rate of approximately 10mL/minute. The beaker was also simultaneously stirred as the CaCl2 was poured in. Once combined, a thermometer was used to measure and record the temperature. The mixture was then allowed to sit at temperature for 5 minutes.
By using TLC and IR spectrum, we will see the difference between benzophenone (starting material) and diphenylmethanol (final product). Experimental Procedure: Benzophenone (358mg, 2.0mmol) was dissolved in ethanol (5ml) in a 25ml round bottomed flask. The solid did not completely dissolve, so the flask was swirled with magnetic stirrer bar. In a small test tube, sodium borohydride (87mg, 2.2mmol) was dissolved in cold water (1.5ml). This solution was added drop wise to the stirred ethanolic solution of benzophenone at room temperature.
Whiles the cuvette remains in the machine for the entire 5 minutes and noting the value every 30 seconds as given in the instruction. The process was repeated by using a clean cuvette each time but in the presence of different amount of trypsin inhibitor (stock solution of 0.1mg/ml in buffer).and by putting the mix in a beaker separately to avoid melting/dissolve any
After heating, the mixture was cooled to room temperature and filtered by vacuum filtration into a fritted funnel to yield a purple product. The product was washed 3 times with (5mL) portions of chilled 6M HCL, then Ethanol, and lastly with acetone. The resulting product was placed into a vial and left to dry in a vacuum desiccator for 1 week and weighed the next week. The yield was 6.029g. The second experiment, procedure 1, combined [Co(NH3)5 (H2O)]Cl2 (0.0060M, 1.52g) and (25mL) of distilled water to an 125mL Erlenmeyer flask.
| - | - | 7 | By dissolving the precipitate. | Dark blood red solution was formed. | 6M HCl and 0.1M NH4SCN | Fe3+ + SCN-→ [FeNCS]2+ | 8 Ni2+ | By adding dimethylglyoxime solution. | A red precipitate was formed. | 3 drops of 1% dimethylglyoxime solution.
Add 0.5 ml concentrated HCl and 1.0 ml 15% KI solution. Mixed exactly 1 minute and leave for 5 minutes in a dark place. Add 0.5 ml starch solution, 20 ml distilled water. Mix and titrate with sodium thiosufate solution. Calculate the exact normality of Na2S2O3 knowing that in this chemical reaction 1 gram-equivalent of K2Cr2O7 react with 1 gram-equivalent of Na2S2O3 (1 mole K2Cr2O7 react with 6 moles Na2S2O3).