To Determine the Solute Potential of Plant Cells.

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Title: Water Potential and Osmosis Aim: To determine the solute potential of plant cells Discussion: The aim of this lab was to determine and observe how six different solutions of varying sucrose concentration affected the epidermis of an onion in terms of the solute potential. Onions are made up of 89% water and 8% to 9% soluble sugars, the rest is minerals, fats, proteins, and sulphur compounds. The solute potential (ψs) is the reduction in the water potential of the solution in the cell due the presence of particles dissolved in the solution. The presence of solutes in water causes the solute potential to decrease and it reduces the free energy of water. This is so because water molecules will tend to bind to the solutes rather than moving freely and away from the solution. The small squares of the onion membrane was observed under a light microscope at times forty, after it was left undisturbed in a series of sucrose concentrations for ten minutes. Cells in their cytoplasm contain dilute solutions of sugars protein and salts. If a cell were to be placed in solutions of varying solute concentration in comparison to the cell surroundings, water will either move into the cell or move out of the cell, it all depends on the solution it is placed in. In an isotonic solution the cell will neither gain nor lose water by osmosis. If a plant is placed in a hypotonic solution the water the cell becomes turgid as water diffuses into the cell. In hypertonic solution water will move out of the cell the cell becomes flaccid. The cell undergoes plasmolysis. When the onion cell in distilled water was observed under the light microscope, the cell membrane was pushed up against the walls of the cell wall. The cells were rectangular in shape. The vacuoles of the cells were large. The ψs inside is more negative in comparison to the ψs

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