in nature, the bacteria can transfer these plasmids from bacteria to bacteria, transfering those beneficial genes. this mechanism allows bacteria to adapt to new and foreign environments. the continuous resistance of bacteria to antibiotics is due to the transmission of these plasmids. Bio-Rads unique pGLO plasmid encodes the gene for GFP and a gene for resistance the antibiotic ampicillin. pGLO can also control the gene expression of this fluorecent protein.
Purpose In this lab we are trying to get a broader understanding of the transformation of bacteria by exposing them to pBLU plasmids. Introduction Transformation is the manipulation of a bacterial cell's DNA in order to alter the cell's genotype or phenotype by absorbing free DNA from its surroundings. This can result in a nonpathogenic bacteria becoming pathogenic by absorbing the DNA of a broken open or dead pathogenic bacteria. In our case it is taking in the pBLU plasmid. A plasmid is a spherical self-replicating DNA molecule that is not actually a part of the bacterial cell but can integrate itself into the bacterial chromosome.
I. Title: pGLO Transformation Lab II. Purpose; We want to be able to observe, comprehend and analyze genetic transformation as we genetically alter organisms. III. Hypothesis: If bacteria with + pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies with survive and grow on the transformation plates that have LB/ amp.
Cellular Structure and Pathogenicity Student’s name: Institution: Instructor: Subject: Cellular structure and Pathogenicity A pathogen is a micro organism that is capable of causing ailment to insect, animal or plant (Staskawicz etal, 1987). The ability of the pathogen to create and disease that affects living thing is called pathogenicity. The pathogenicity of pathogen is majorly related to the cellular structure of bacteria. The cellular structure is the key determinant on the adaptation which the bacteria take in order to survive in the host living thing. Basically, there are three major forms of shape that the cells of bacterial can take form in.
Ergogenic Aids in Enhancing Sports Performance Introduction: With heater dope testing methods and hence the possibilities of detection and life can from the sport, athletes and coaches are looking for legal ways to improve performance and / or hasten recovery. The various ways by which performance can be improved are known as organic aids. Ergogenic aids are any external influences, which can positively affect physical or mental performance. Ergogenic Aids may: • Directly influence the physiological capacity of a particular body system thereby improving performance. • Remover psychological Constraints which impact performance.
The reason this makes Koch so important is because if he hadn’t investigated Anthrax using Pasteur’s theory, the germ theory may never have caught on leaving people to believe false theories for much longer. Koch helped the advancement of medicine and life expectancy take a big step forward. Koch used very interesting methods that enabled him to research bacteria much easier than what other scientists researching the subject could. He used certain ways of staining bacteria so they could be photographed; he did this by using dyes such as methyl violet. He also discovered a way of growing bacteria on potatoes rather than in water, meaning they were easier to see and study, as the solid particles didn’t move, unlike the loose water molecules which moved along with the bacteria.
placed in a spectrophotometer. The data collected was used to view the growth of the bacteria and can be used to compare the growth of this E. coli strain to other E. coli strain, bacteria and the general bacterial growth curve. The data collected can make a template to possibly classify unknown bacteria. Not all bacteria replicate and grow at the same rate. Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis.
Biological chelation therapy - The process which involve the use of chelating agents to remove heavy metals from the source. Objectives The aim of this article is to know and learn about the importance of using of biological chelation therapy agents. This article is also allowing people to know what exactly biological chelation therapy agents are and how they can be used to improve our life. As it gives people the importance, it also let people to be aware about side effects of biological chelation therapy agents. This article also tells people about the background of biological chelation therapy agents in such way that where they come from or where they are produced and if they suitable to be used by human beings.
Gene therapy is a promising approach to the treatment of genetic disorders, debilitating neurological diseases such as Parkinson’s and endocrine disorders such as diabetes. This technology may therefore yield profound benefits for human health and quality of life (Medical News Today, 2004). Genomics tools have potential to make significant impacts on the area of environmental monitoring, better understanding of complex ecosystems, and basic science discovery of new species in our oceans, soil and air (J. Craig Venter Institute, 2005). The dangers of biosynthesized systems stems from the ability of biological processes to easily support self-replication. Some observers believe that self-replicating itself should be strictly banned as the only way to fend off the threat of some engineered molecular system running rampant (Brown, 2004).
The Action Spectrum and the Absorption Spectrum Introduction: The purpose of this experiment is to learn the rates of photosynthesis under different lights, and the absorbance of photosynthetic pigments in spinach extract. “There would be no biology without photosynthesis. Plant biomass is the food and fuel for all animals. Plants are the primary producers. These amazing organisms are capable of capturing the energy of sunlight and fixing it in the form of potential chemical energy in organic compounds.” (Measuring the rate of photosynthesis) “Light is one of the main factors that affects the rate of photosynthesis, which literally means using light to create something new.” (What Are Three Factors That Affect the Rate Which Photosynthesis Occurs?)