The Effect of Temperature on Membranes

1034 Words5 Pages
The effect of temperature on membranes HYPOTHESIS: An increase in temperature, the more the red dye anthocyanin will be released from the beetroot. This will also damage and denature the plasma membrane causing the cytoplasm and other substances contained within the membrane to escape out. VARIABLES: The IV: the temperature, which will be measured in degrees Celsius. The DV: the amount of red dye anthocyanin, which leaks out of the cell membrane. This will be measured in percentage transmission. REULTS % ABSORBACE TEMP | 1 | 2 | 3 | 30 | 0.43 | 0.40 | 0.45 | 40 | 0.49 | 0.44 | 0.74 | 50 | 1.36 | 0.80 | 1.00 | CONCLUSION Overall the graph and table of results does show a positive correlation between the increase of temperature and the increase of release of red pigment (increase in light absorption). Therefore I believe my hypothesis is very correct. This is in terms of where the increase of heat (temperature is applied) onto the beetroot pieces, there is an increase in the release of red pigment. The graph shows the results from the three tests taken, each at 30, 40 and 50 °C. I added the anomalies in the graph as well to give an overview of the difference in each test. By looking at the results, I can see that in test 1, at 50 °C, the result said 1.36 which is a very large jump from 0.43 at 30 °C, and 0.49 at 40 °C. This is an anomaly. Another anomaly is in test 3, at 40 °C, the result said 0.74 from a lower 0.45. At first as the increase of temperature is applied, from 30 °C to 40 °C, I would not expect any of the components of the membranes to have become damaged or denature. This is because I believe that these temperatures are not high enough to damage or denature the proteins and/ or lipids. What I believe has caused the increase of red pigment is the increase of kinetic energy due to the increase of
Open Document