The Effect of Enzyme Concentration on the Activity of Catalase

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EXPERIMENT: THE EFFECT OF ENZYME CONCENTRATION ON THE ACTIVITY OF CATALASE DESIGN Aim To investigate the relationship between the enzyme concentration and the activity of catalase. Research Question How does the rate of oxygen production by the decomposition of hydrogen peroxide vary with the increasing concentration of the enzyme catalase? Hypothesis The rate of oxygen production increases as the concentration of the micro-organism yeast, which is the source of the enzyme, increases. Variables Independent Variable: Concentration of yeast Dependent Variable: Rate of oxygen production Controlled Variables: Volume of hydrogen peroxide, mass of hydrogen peroxide, temperature and pressure Apparatus • Instant dry yeast • Measuring cylinder • Boiling tube • Delivery tube • Stopper • Pipette • 5 cm3 hydrochloric acid • 100 cm3 beakers • Stopwatch Warning Hydrogen peroxide may cause burns to skin and clothing. Wash off any spillages using plenty of water. Method 1. Stir the yeast and place in the boiling tube. 2. Put the stopper into the boiling tube but do not place the delivery tube under the measuring cylinder yet. 3. Fill the syringe with 1 ml 10% hydrogen peroxide and attach to the syringe needle. 4. Add the hydrogen peroxide to the yeast suspension. Place the delivery tube under the measuring cylinder and start a stopwatch. 5. Record the volume of oxygen collected every minute until no more is produced or until the measuring cylinder is full. 6. Repeat with different concentrations of yeast. (Maximum 2 gr of yeast) 7. Record your results in a table. 8. Calculate the rate of oxygen production for each concentration and plot your results as a graph. DATA COLLECTION AND PROCESSING Mass of yeast / g Initial volume / cm3 Final volume / cm3 Volume difference / cm3 0.25 50 62 12 0.50 50 69 19 0.75

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