Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Solutions: distilled water, 40% glucose solution, 10% NaCl solution and 40% sucrose solution. Also we had equipment and supplies for Benedict’s test and AgNO3 test, such as Benedict’s solution, silver nitrate and beaker with boiling water. 1. Previously number marked beakers half feel with distilled water except #2, which has to be filled with 40% glucose solution. 2.
From the gel electrophoresis, we found that the PCR product was about 600 bps. Spectrometric analysis helped us to find the concentration of amplified DNA which was about 65 μL/mL. The next step was to produce digoxigenin-UTP labelled RNA probe by in vitro transcription of PCR amplified tau DNA with T7 RNA polymerase. About 4 μL of template DNA was added to a sterile, RNase-free reaction vial. The total sample volume was made up to 13 μL by adding water.
In 1970, the Federal Bureau of Investigation developed a computerized automated fingerprinting system, called AFIS, which is still in use today. Sir Alec Jeffreys developed the first DNA profiling technique in England in 1985. Fhe FBI's combined DNA index system, or CODIS, which allowed different crime labs and departments to share DNA information, was created in 1990. In the early 2000s, the Scanning Kelvin Probe was developed by scientists at the Swansea University in the United Kingdom. The probe uses electric voltage to "see" latent fingerprints, even on surfaces that have been wiped
When the door trim on the child’s closet was pulled out, the former telephone number and address of John Condon were written in pencil (Petersen). Handwriting analysis found that Hauptmann’s penmanship was stylistically consistent with the ransom notes that were sent by the kidnapper or kidnappers. He drew additional suspicion for having a prior criminal record that included burglary (Lindbergh Baby Kidnapping). On October 10, 1934 a New Jersey grand jury indicts Hauptmann on murder and kidnapping charges (Busch). The trial was set for January 2, 1935 and it was expected to be the trial of the century (Petersen).
NOTE: A minimum of FIVE APA sources are required in reference list and five seperate in-Text application of sources. Collaborate as a mock forensic team. Prepare a 12- to 16-slide Microsoft® PowerPoint® presentation in addition to a chain of custody that proposes forensic evidence to be collected and analyzed in investigating one of the following hypothetical cases: A murdered 18-year old female victim found on the bank of a river A sexually assaulted 30-year old female victim who self-admitted to the emergency room An abused 5-year old male victim investigated by Child Protective Services An abused 77-year old male victim investigated by Adult Protective Services A 24-year deceased female victim of a hit-and-run vehicle accident A house fire that contains the remains of a 45-year old father, 43-year old mother, 11-year old son, and 9-year old
Assignment #7 Who Hit Reveille? Vinay Jithendran 11/11/2013 BIO 111-502 Lab Lab Instructor: Matthew Markert Abstract: The examination of evidence, taken from Reveille’s hit and run accident, aided in the investigation of the perpetrator who did so. DNA, fingerprint, blood, and hair evidence was taken from the scene of the crime and compared to the testimonies of the suspects, Bikerman and Maroon Flash. The DNA evidence of the two suspects were observed, through gel electrophoresis, which produces a DNA fingerprint of the culprit. Fingerprint evidence was observed, through specific patterns in the fingerprints.
DNA Fingerprinting: Revealing the Truth Name: Zainab Khatoon Due Date: March 19th, 2012 BSC2010L.019S12 Course Name: Biology I Cellular Processes Lab Section: 019 Lab Partner: Hiba Fatima Materials and Methods Restriction Enzyme Digestion Procedure: As with all experiments done in this lab, preparation was accounted for with gloves and goggles. To begin the restriction enzyme digestion procedure, microtest tubes were labeled 1 through 4 for different reactions of restriction enzyme digestion. Then, with the use of the P20 micropipette, ten microliters of Enzyme Reaction Buffer were dispensed into each of the reaction tubes. After the Buffer was dispensed, a fresh micropipette tip replaced the old one. The tip of the micropipette
What physical evidence was related to the case? The main physical evidence used to solve this case was the biological DNA evidence from the vaginal swabs from the three elderly women who had been raped. (Saferstein, R. 2009) 4. What was the outcome of the case? In April, 2001 convicted-offender database got what was called a “cold-hit” because the perpetrator of the crime had been convicted of shooting at a residence that was occupied, which in North Carolina requires that the persons DNA be placed in the criminal database.
At the scene the detective finds torn par of underpants with white residue on them. He bags and tags the evidence and transports it directly to the lab for evaluation to try and find traces of DNA. If DNA is found they may have the person in the Database already. The detective then goes to the hospital to talk with the victim to get her story. He asks her question about where she was coming from, what the person looked like, if she was with anyone at the club, and if she could have been followed from the club.