Structured Model On Penicillin Production

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ELSEVIER Biochemical Engineering Journal 2 ( 1998) Biochemical Engineering Journal 1 l-21 A structured model for penicillin production on mixed substrates G.C. Paul, M.T. Syddall, C.A. Kent, C.R. Thomas * Centre for Bioprocess Engineering, School of Chemical Received Engineering, 23 January The University of Birmingham, 1998; accepted 7 May Edgbaston, Birmingham, B15 2lT, UK 1998 Abstract A structured kinetic modelpreviouslydeveloped describe growth,differentiation, penicillinproduction Penicillium chryysoto the and of genum has been enhanced andextended orderto apply it to a mixedcarbonsource fermentation. filamentous in The hyphae dividedinto are four distinctregions thebasis theiractivitiesandthephysiological on of structure (i.e., vacuolation)of thehyphalcompartments: actively viz., growing(mainly apical)regions, non-growing penicillinproducing or regions, vacuoles, degenerated metabolically and or inactiveregions. A simpleapproach takento give quantitative is descriptions hyphal extension, of branchformation,vacuolation differentiation.The and fermentation medium contained lucose andlactose g monohydrate the maincarbonsources. The source the lactose whey powder as of was used in excess in theinoculum medium, whilstglucose fedcontinuously was throughout fermentation. the Lactose,disaccharide, is hydrolysed a to two monosaccharides, glucose and gaiactose, when the residual glucose concentration in the medium drops to a very low level. The utilisationof glucose that of galactose and following the hydrolysisof lactosewereobserved occursimultaneously. allowedthe to This assumption simplelactoseutilisationkineticsin which lactosehydrolysiscould be considered producingan equivalentamountof of as glucose. model been sed for successful The has u

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