Your answer: yes;results showed they have partial identity Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Lab Report 1. Describe how the direct and indirect ELISA are different. Your answer: Direct ELISA is directly looking for the foreign substance. The microtiter plate is coated with homologous antibodies made against the antigen of interest If the antigen is present, a sandwich of antibody, antigen and secondary antibody will form Indirect ELISA is designed to detect antibodies that the patient has made against the antigen.The microtiter plate is coated with
In Microbiology Lab 3 I chose the unknown culture #14 and ultimately identified it as Staphylococcus epidermidis bacteria. On a microscopic level I found the organism to be gram positive with a coccobacillus shape and both tetrad and cluster cell arrangement. I performed an isolation streak with S. epidermidis on Nutrient Agar which resulted in pinpoint, round, entire, and flat macroscopic morphology. I took a loopful of the organism from the Nutrient Agar and placed it on a slide to perform the catalase test. I added a few drops of 3% Hydrogen Peroxide and it resulted in bubble formation.
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
Mitosis and Meiosis Lab Purpose: The purpose of this lab is to observe and diagram the various phases of mitosis and meiosis using plant and animal cells. Means of Accomplishment: I accomplished this lab by following the instructions provided to me by my lab format worksheet and from my lab advisor, Ms. Hieter. First, I went to my lab setup, where a microscope and one of the prepared onion and fish slides were available. I searched and observed for each of the four phases in the whitefish slide for meiosis and drew a diagram for each of these phases. Then, I searched for the four phases in the onion root tip slide for the processes of mitosis and drew a diagram each one.
Also the position of the bands tells you the comparison between the weights of the molecules within the sample. The stacking gel is needed to pack all the proteins in one band, so that they will start migrating in the running gel all at the same time. In the stacking gel the solutions added were acrylamide, 1 M Tris HCl pH 6.8, SDS, distilled water, APS, and TEMED. The resolving gel allows separating the proteins in the samples based on their weights. Analyze the bands in the gel: 1) 1 band- 1 subunit (monomer)- Integrase - degraded protein under the band with low intensity 2) 1 thick band- two identical subunits- beta complex- ran as a single band, the more concentration the thicker the band is going to look.
During the PCR reaction, the forward and reverse primers anneal to the complementary sequence on the DNA and a probe that is labelled with a 5’ dye and a 3’ quencher, anneals to the target gene of the bacterium’s DNA. During PCR amplification. The probe is then cleaved and the 5’ dye is then separated from the 3’ quencher, allowing the dye to emit fluorescence. Emission of fluorescence at elongation indicate the presence of the target
DNA Fingerprinting: Revealing the Truth Name: Zainab Khatoon Due Date: March 19th, 2012 BSC2010L.019S12 Course Name: Biology I Cellular Processes Lab Section: 019 Lab Partner: Hiba Fatima Materials and Methods Restriction Enzyme Digestion Procedure: As with all experiments done in this lab, preparation was accounted for with gloves and goggles. To begin the restriction enzyme digestion procedure, microtest tubes were labeled 1 through 4 for different reactions of restriction enzyme digestion. Then, with the use of the P20 micropipette, ten microliters of Enzyme Reaction Buffer were dispensed into each of the reaction tubes. After the Buffer was dispensed, a fresh micropipette tip replaced the old one. The tip of the micropipette
Title: Introduction to agrarose gel electrophoresis. Name: D Creaven Date: 4th February 2013 Module: Forensic DNA Laboratory Tutor: Dr Colin Conway Title Introduction to agrarose gel electrophoresis. Date 4th February 2013 Lab Partners Jurgita Baltrukoniene Janet McHugh Objective (a) To prepare the agrarose gel electrophoresis (b) To analysis the movement of DNA on the agrarose gel. Introduction Agarose gel electrophoresis is an easy way to separate DNA fragments by their sizes and visualize them. It is a common diagnostic procedure used in molecular biological labs.
I have chosen to investigate the most common type of DNA profiling, restriction fragment length polymorphism (RFLP). The first part of the process is called restriction digest. Firstly the DNA is broken into pieces using an enzyme called Hinfl. This enzyme, which comes from the bacteria Haemophilus influenza, is able to recognise and break DNA whenever a sequence of GANTC appears. GANTC stands for the bases that are found in DNA (Guanine, Adenine, Cytosine and Thymine).
Protein | M.W. | pI | Label | Ex. Max | Em. Max | Color | CYC | 13,400 | 10.6 | Heme | 390,528 | - | Red | Alpha-LA | 14,280 | 4.4 | FITC | 495 nm | 528 | Yellow | BSA | 68,500 | 4.9 | FITC | 495 nm | 528 | Yellow | pBSA | ~68,500 | >7.0 | TRITC | 520,540 | 582 | Purple | Purpose: The purpose of this experiment is to gain an understanding of how chemical and physical properties allow for the separation of a mixture of proteins using principles in chromatography.