The cap of a centrifuge tube was used to collect approximately one (1) gram of soil from the sample. This was then added to a bottle containing 99ml of sterile water for a 10-2 dilution, 1ml of 10-2 dilution was then added to a bottle containing 99ml of water for a 10-4 dilution. 1ml from the 10-4 dilution was then added to 99ml of water for a final dilution of 10-6. 1ul of each was plated on different starch agar plates with the aid of a micropipette and a plate spreader. The plates were then incubated for a week.
5. After 5 minutes I removed the cabbage from the stove and carefully drained the broth into a new glass bowl. 6. I allowed the broth to cool for a 30 minute period. 7.
We used Polymerase Chain Reaction to amplify samples of the bacterial plasmid DNA. This allowed us to determine whether the three farms have a common form of bacteria that is resistant to tetracycline. In petri dishes each member of the team used dilution of 10-2 to 10-6. With a pipette we put 100μl sample of bacteria. The dishes were placed in an incubator for 24 hours then kept in a cold room until ready for the count of bacteria.
We now slowly pour the solution into a funnel with filter paper. The extract along with 2 pieces of Iodine are added to a new beaker and left for 10 minutes. Finally to determine if the lab was successful, three tests are conducted for Iodine, Iodide and triiodide. The objective is to produce a tincture of iodine by extracting iodide and other components from seaweed. Warm up Activity 1.
We first labeled each cup with what type of water we were going to use. We put an equal amount of soil into each cup. Our next step was to put exactly 20 seeds into each cup approximately ¼ inch deep. After that we poured 20ml of each type of water into its designated cup. After the grass started to sprout we measured its growth in centimeters and then gave each cup its 20ml of water.
Purpose: The purpose of this lab is to prepare and purify a fuel, ethanol C2H5OH. We will learn to do so by the process of fermentation, and distillation. Over the course of 2-3 weeks, we will be collecting and analyzing the data for this lab. Hypothesis: If the alcohol ferments correctly, then 75% alcohol will be produced with a volume on 150 mL out of the initial 200 mL solution. Procedure: Refer to Chemistry Lab Manual pp.
and Weed control experts. Hypothesis If home-made and commercial herbicides are sprayed on weeds then the commercial herbicides will be more effective, because the chemicals are made by experts who know what chemicals kill and prevent weeds. Experiment Fill 2 different boxes and fill then with soil make sure to use the same type and amount of soil. Measure around one cup of grass seed and sprinkle it on the soil. Water the seeds daily until the grass is at least 1.5 inches tall.
In 1989, EPA removed approximately 70,000 cubic yards of shingles from the Bay Drum site in order to evaluate the extent of soil contamination. The EPA conducted a second removal action in 1990 and removed contaminated soils, drums of hazardous waste, and bags of pesticides from the site. Clean up on OU1 and OU3 began in 2000 and were completed in 2001. Implementation of the amended clean up of OU3 began in the Spring of 2005 and the ground water data indicates that the vegetable oil was performing as expected and will likely be in place for a considerable period of
Hypothesis: Corresponding to the theory of composting, it is more than possible that a cup filled with organic matter, leaves, water, and soil will be decomposed from at the end of a fourteen-day period, producing and organic mixture rich in nutrients, that will help the nourishment of my garden, and small orchard in my mom's backyard. • Plastic Cup • Scissors • Plastic wrap • Camera • thermometer • Notebook • Vegetable scraps • Fruit scraps • Egg shell • Soil • Grass clippings • Leaves • Water Procedure: Made holes on the bottom of the plastic cup with a scissors, and placed of the organic matter on a bowl and mixed with a plastic spoon. At the end, I made a little hole on the plastic wrap and introduced the thermometer on the middle of the cup. Experiment: • I placed the plastic cup on my kitchen window where direct sunlight and shade is accessible to prevent extreme temperatures. This composting lab.
After, pour the exact amount of pollutants into the soil in the cup. Finally, let the soil and pollutants stand in the filter for one minute. The results had dirt holding 49.7 ml of gas, 133.6 ml of oil, and 62.4ml of turpentine. Sand held 13.6ml of gas, 94.1ml of oil, and 21.7ml of turpentine. Clay held 93.8ml of gas, 146.2ml of oil, and 104.5ml of turpentine.